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Beyond the Roles in Biomimetic Chemistry: An Insight into the Intrinsic Catalytic Activity of an Enzyme for Tumor-Selective Phototheranostics
ACS Nano ( IF 15.8 ) Pub Date : 2018-11-12 00:00:00 , DOI: 10.1021/acsnano.8b05797 Weitao Yang 1 , Xiudong Shi 2 , Yuxin Shi 2 , Defan Yao 1 , Shizhen Chen 3 , Xin Zhou 3 , Bingbo Zhang 1
ACS Nano ( IF 15.8 ) Pub Date : 2018-11-12 00:00:00 , DOI: 10.1021/acsnano.8b05797 Weitao Yang 1 , Xiudong Shi 2 , Yuxin Shi 2 , Defan Yao 1 , Shizhen Chen 3 , Xin Zhou 3 , Bingbo Zhang 1
Affiliation
Protein-assisted biomimetic synthesis has been an emerging offshoot of nanofabrication in recent years owing to its features of green chemistry, facile process, and ease of multi-integration. As a result, many proteins have been used for biomimetic synthesis of varying kinds of nanostructures. Although the efforts on exploring new proteins and investigating their roles in biomimetic chemistry are increasing, the most essential intrinsic properties of proteins are largely neglected. Herein we report a frequently used enzyme (horseradish peroxidase, HRP) to demonstrate the possibility of enzymatic activity retaining after accomplishing the roles in biomimetic synthesis of ultrasmall gadolinium (Gd) nanodots and stowing its substrate 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt) (ABTS), denoted as [email protected]ABTS. It was found that ca. 70% of the enzymatic activity of HRP was preserved. The associated changes of protein structure with chemical treatments were studied by spectroscopic analysis. Leveraging on the highly retained catalytic activity, [email protected]ABTS exerts strong catalytic oxidation of peroxidase substrate ABTS into photoactive counterparts in the presence of intrinsic H2O2 inside the tumor, therefore enabling tumor-selective catalytic photoacoustic (PA) imaging and photothermal therapy (PTT). In addition, the MR moiety of [email protected]ABTS provides guidance for PTT and further diagrams that [email protected]ABTS is clearable from the body via kidneys. Preliminary toxicity studies show no observed adverse effects by administration of them. This study demonstrates beyond the well-known roles in biomimetic chemistry that HRP can also preserve its enzymatic activity for tumor catalytic theranostics.
中文翻译:
在仿生化学中的作用之外:肿瘤选择性光热酶的内在催化活性的见解。
近年来,由于蛋白质辅助仿生合成具有绿色化学,工艺简便和易于集成等特点,因此已成为纳米加工的新兴分支。结果,许多蛋白质已用于仿生合成各种纳米结构。尽管探索新蛋白质并研究其在仿生化学中的作用的努力正在增加,但是蛋白质的最基本内在特性却被大大忽略了。在这里,我们报告了一种常用的酶(辣根过氧化物酶,HRP),以证明在完成仿生合成超小g(Gd)纳米点的作用并将其底物2,2'-氮杂双(3-乙基苯并噻唑啉- 6磺酸铵盐(ABTS),表示为[电子邮件保护]ABTS。结果发现,CA。保留了70%的HRP酶促活性。通过光谱分析研究了化学处理引起的蛋白质结构的相关变化。利用高度保留的催化活性,[受电子邮件保护] ABTS在肿瘤内部存在内在H 2 O 2的情况下,将过氧化物酶底物ABTS强烈催化氧化成光活性对应物,从而实现肿瘤选择性催化光声(PA)成像和光热治疗(PTT)。此外,[受电子邮件保护的] ABTS的MR部分为PTT提供了指导,并提供了可以从身体清除[受电子邮件保护的] ABTS的其他图表。通过肾脏。初步毒性研究表明,未观察到不良反应。这项研究证明了仿生化学中众所周知的作用,即HRP还能保留其对肿瘤催化治疗的酶活性。
更新日期:2018-11-12
中文翻译:
在仿生化学中的作用之外:肿瘤选择性光热酶的内在催化活性的见解。
近年来,由于蛋白质辅助仿生合成具有绿色化学,工艺简便和易于集成等特点,因此已成为纳米加工的新兴分支。结果,许多蛋白质已用于仿生合成各种纳米结构。尽管探索新蛋白质并研究其在仿生化学中的作用的努力正在增加,但是蛋白质的最基本内在特性却被大大忽略了。在这里,我们报告了一种常用的酶(辣根过氧化物酶,HRP),以证明在完成仿生合成超小g(Gd)纳米点的作用并将其底物2,2'-氮杂双(3-乙基苯并噻唑啉- 6磺酸铵盐(ABTS),表示为[电子邮件保护]ABTS。结果发现,CA。保留了70%的HRP酶促活性。通过光谱分析研究了化学处理引起的蛋白质结构的相关变化。利用高度保留的催化活性,[受电子邮件保护] ABTS在肿瘤内部存在内在H 2 O 2的情况下,将过氧化物酶底物ABTS强烈催化氧化成光活性对应物,从而实现肿瘤选择性催化光声(PA)成像和光热治疗(PTT)。此外,[受电子邮件保护的] ABTS的MR部分为PTT提供了指导,并提供了可以从身体清除[受电子邮件保护的] ABTS的其他图表。通过肾脏。初步毒性研究表明,未观察到不良反应。这项研究证明了仿生化学中众所周知的作用,即HRP还能保留其对肿瘤催化治疗的酶活性。