How do Enzymes Orient when Trapped on Metal-Organic Framework (MOF) Surfaces?,Journal of the American Chemical Society

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How do Enzymes Orient when Trapped on Metal-Organic Framework (MOF) Surfaces?
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2018-11-12 , DOI: 10.1021/jacs.8b09257
Yanxiong Pan ₁ , Hui Li ₁ , Jasmin Farmakes ₁ , Feng Xiao ₂ , Bingcan Chen ₃ , Shengqian Ma ₄ , Zhongyu Yang ₁

Affiliation

Enzyme immobilization in metal-organic frameworks (MOFs) offers retained enzyme integrity and activity, enhanced stability, and reduced leaching. Trapping enzymes on MOF surfaces would allow for catalysis involving large substrates. In both cases, the catalytic efficiency and selectivity depend not only on enzyme integrity/concentration but also orientation. However, it has been a challenge to determine the orientation of enzymes that are supported on solid matrices, which is even more challenging for enzymes immobilized/trapped in MOFs due to the interferences of the MOF background signals. To address such challenge, we demonstrate in this work the utilization of site-directed spin labeling in combination with Electron Paramagnetic Resonance spectroscopy, which allows for the first time the characterization of the orientation of enzymes trapped on MOF surfaces. The obtained insights are fundamentally important for MOF-based enzyme immobilization design and understanding enzyme orientation once trapped in solid matrices or even cellular confinement conditions.

中文翻译：

当被困在金属有机框架 (MOF) 表面时，酶如何定向？

金属有机框架 (MOF) 中的酶固定化提供了保留的酶完整性和活性、增强的稳定性和减少的浸出。在 MOF 表面捕获酶将允许涉及大底物的催化。在这两种情况下，催化效率和选择性不仅取决于酶的完整性/浓度，还取决于方向。然而，确定支持在固体基质上的酶的方向一直是一个挑战，由于 MOF 背景信号的干扰，这对于固定/捕获在 MOF 中的酶更具挑战性。为了应对这样的挑战，我们在这项工作中展示了定点自旋标记与电子顺磁共振光谱相结合的利用，这首次允许表征捕获在 MOF 表面上的酶的方向。所获得的见解对于基于 MOF 的酶固定化设计和理解一旦陷入固体基质甚至细胞限制条件中的酶方向至关重要。

更新日期：2018-11-12

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