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Rapid Detection of Severe Fever with Thrombocytopenia Syndrome Virus via Colloidal Gold Immunochromatography Assay
ACS Omega ( IF 3.7 ) Pub Date : 2018-11-13 00:00:00 , DOI: 10.1021/acsomega.8b02366 Jia-Ying Zuo 1 , Yong-Jun Jiao 2 , Jin Zhu 3 , Shou-Nian Ding 1
ACS Omega ( IF 3.7 ) Pub Date : 2018-11-13 00:00:00 , DOI: 10.1021/acsomega.8b02366 Jia-Ying Zuo 1 , Yong-Jun Jiao 2 , Jin Zhu 3 , Shou-Nian Ding 1
Affiliation
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To develop the point-of-care testing method to facilitate the clinical detection of severe fever with thrombocytopenia syndrome virus (SFTSV), colloidal gold paper-based lateral flow immunochromatography test strips (LFITSs) have been fabricated for the rapid detection for the first time. The pH value and the amount of monoclonal antibody to prepare colloidal gold nanoparticle-labeled monoclonal antibody bioconjugates were optimized. In addition, 0.4% bovine serum albumin was considered to be the best concentration for blocking nitrocellulose membranes. Under optimal conditions, the limit of detection for SFTSV was as low as 1 ng/mL depending on a visual line. Meanwhile, the entire detection process required no more than 10 min with a volume of only 50 μL of the analyte solution. Moreover, paper-based LFITSs were evaluated in real samples of human serum of patients with satisfactory results. In addition, all strips were of high stability and specificity. In the light of advantages such as simple, portable, rapid, and low cost, the developed LFITSs will extensively come into service, especially in remote areas.
中文翻译：
通过胶体金免疫层析法快速检测血小板减少综合征病毒的严重发烧
为了开发床旁检测方法以方便临床检测严重发热伴血小板减少综合征病毒（SFTSV），首次制备了胶体金纸侧流免疫层析试纸条（LFITS）用于快速检测。对制备胶体金纳米颗粒标记的单克隆抗体生物结合物的pH值和单克隆抗体的量进行了优化。另外，0.4%牛血清白蛋白被认为是封闭硝酸纤维素膜的最佳浓度。在最佳条件下，SFTSV 的检测限低至 1 ng/mL，具体取决于视线。同时，整个检测过程不超过10分钟，分析物溶液体积仅为50μL。此外，在患者的真实人血清样本中对纸基 LFITS 进行了评估，取得了令人满意的结果。此外，所有试纸条均具有高稳定性和特异性。所开发的LFITS具有简单、便携、快速、成本低等优点，将广泛投入使用，特别是在偏远地区。
更新日期：2018-11-13
中文翻译：
通过胶体金免疫层析法快速检测血小板减少综合征病毒的严重发烧
为了开发床旁检测方法以方便临床检测严重发热伴血小板减少综合征病毒（SFTSV），首次制备了胶体金纸侧流免疫层析试纸条（LFITS）用于快速检测。对制备胶体金纳米颗粒标记的单克隆抗体生物结合物的pH值和单克隆抗体的量进行了优化。另外，0.4%牛血清白蛋白被认为是封闭硝酸纤维素膜的最佳浓度。在最佳条件下，SFTSV 的检测限低至 1 ng/mL，具体取决于视线。同时，整个检测过程不超过10分钟，分析物溶液体积仅为50μL。此外，在患者的真实人血清样本中对纸基 LFITS 进行了评估，取得了令人满意的结果。此外，所有试纸条均具有高稳定性和特异性。所开发的LFITS具有简单、便携、快速、成本低等优点，将广泛投入使用，特别是在偏远地区。
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