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Selective and Sensitive Sensing of Hydrogen Peroxide by a Boronic Acid Functionalized Metal–Organic Framework and Its Application in Live-Cell Imaging
Inorganic Chemistry ( IF 4.3 ) Pub Date : 2018-11-08 00:00:00 , DOI: 10.1021/acs.inorgchem.8b02240
Mostakim SK 1 , Sooram Banesh 2 , Vishal Trivedi 2 , Shyam Biswas 1
Inorganic Chemistry ( IF 4.3 ) Pub Date : 2018-11-08 00:00:00 , DOI: 10.1021/acs.inorgchem.8b02240
Mostakim SK 1 , Sooram Banesh 2 , Vishal Trivedi 2 , Shyam Biswas 1
Affiliation
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A new boronic acid functionalized Zr(IV) metal–organic framework having the capability of sensing H2O2 in live cells is reported. The Zr-MOF bears a UiO-66 structure and contains 2-boronobenzene-1,4-dicarboxylic acid (BDC-B(OH)2) as a framework linker. The activated Zr-UiO-66-B(OH)2 compound (called 1′) is highly selective for the fluorogenic detection of H2O2 in HEPES buffer at pH 7.4, even in the presence of interfering ROS (ROS = reactive oxygen species) and other biologically relevant analytes. The fluorescent probe was found to display extraordinary sensitivity for H2O2 (detection limit 0.015 μM) in HEPES buffer, which represents a lower value in comparison to those of the MOF probes documented so far for sensing H2O2 using other analytical methods. Taking advantage of its high selectivity and sensitivity for H2O2 in HEPES buffer, the probe was successfully employed for the imaging of intracellular H2O2. Imaging studies with MDAMB-231 cells revealed the emergence of bright blue fluorescence after loading with probe 1′ and subsequent treatment with H2O2 solution.
中文翻译：
硼酸官能化的金属有机骨架对过氧化氢的选择性和灵敏性及其在活细胞成像中的应用
报道了一种新型的硼酸官能化的Zr（IV）金属有机框架，具有检测活细胞中H 2 O 2的能力。Zr-MOF具有UiO-66结构，并包含2-硼硼苯-1,4-二羧酸（BDC-B（OH）2）作为骨架连接基。活化的Zr-UiO-66-B（OH）2化合物（称为1'）对HEPES缓冲液中pH 7.4的H 2 O 2的荧光检测具有高度选择性，即使在存在干扰ROS（ROS =反应性氧）的情况下种）和其他与生物有关的分析物。发现该荧光探针对H 2 O 2表现出非凡的敏感性（检测限0.015μM）在HEPES缓冲液中，与迄今为止记录的使用其他分析方法感测H 2 O 2的MOF探针相比，该值较低。利用其在HEPES缓冲液中对H 2 O 2的高选择性和敏感性，该探针成功用于细胞内H 2 O 2的成像。用MDAMB-231细胞进行的成像研究显示，在装入探针1'和随后用H 2 O 2溶液处理后，出现了亮蓝色的荧光。
更新日期：2018-11-08
中文翻译：
硼酸官能化的金属有机骨架对过氧化氢的选择性和灵敏性及其在活细胞成像中的应用
报道了一种新型的硼酸官能化的Zr（IV）金属有机框架，具有检测活细胞中H 2 O 2的能力。Zr-MOF具有UiO-66结构，并包含2-硼硼苯-1,4-二羧酸（BDC-B（OH）2）作为骨架连接基。活化的Zr-UiO-66-B（OH）2化合物（称为1'）对HEPES缓冲液中pH 7.4的H 2 O 2的荧光检测具有高度选择性，即使在存在干扰ROS（ROS =反应性氧）的情况下种）和其他与生物有关的分析物。发现该荧光探针对H 2 O 2表现出非凡的敏感性（检测限0.015μM）在HEPES缓冲液中，与迄今为止记录的使用其他分析方法感测H 2 O 2的MOF探针相比，该值较低。利用其在HEPES缓冲液中对H 2 O 2的高选择性和敏感性，该探针成功用于细胞内H 2 O 2的成像。用MDAMB-231细胞进行的成像研究显示，在装入探针1'和随后用H 2 O 2溶液处理后，出现了亮蓝色的荧光。
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