Cellular Uptake and Intracellular Antioxidant Activity of Zein/Chitosan Nanoparticles Incorporated with Quercetin,Journal of Agricultural and Food Chemistry

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Cellular Uptake and Intracellular Antioxidant Activity of Zein/Chitosan Nanoparticles Incorporated with Quercetin
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-11-08 00:00:00 , DOI: 10.1021/acs.jafc.8b04571
Juan-Juan Ma ₁ , Yi-Gang Yu _{1,

2} , Shou-Wei Yin _{1,

2} , Chuan-He Tang _{1,

2} , Xiao-Quan Yang _{1,

2}

Affiliation

In this work, zein/chitosan nanoparticles (ZCPs-Q) were developed for encapsulating quercetin to overcome its lower water solubility and instability, and to concomitantly enhance its cellular uptake and intracellular antioxidant activity. This strategy enhanced quercetin solubility 753.6 and 9.95 times in water and PBS (7.4), respectively, and quercetin encapsulated in ZCPs remained stable after UV irradiation and heat treatment. ZCPs-Q could significantly attenuate AAPH induced erythrocyte hemolysis through the inhibition of ROS generation. It restored intracellular antioxidant enzyme (SOD and GSH-Px) activities to normal levels and inhibited intracellular malondialdehyde (MDA) formation. Simultaneously, ZCPs-Q showed a strong antioxidant activity in HepG2 cells with an EC₅₀ value of 31.18 μg/mL, which was lower than free quercetin’s 41.02 μg/mL. ZCPs enhanced the uptake efficiency of quercetin in Caco-2 cells, which contributed to the improvement of cellular antioxidant activities (CAA) evaluated with the CAA assay and AAPH-induced erythrocyte hemolysis assay. The designed route is particularly suitable for the encapsulation of water-insoluble nutraceuticals and for enhancing cell uptake and CAA.

中文翻译：

槲皮素掺入的玉米醇溶蛋白/壳聚糖纳米颗粒的细胞吸收和细胞内抗氧化活性

在这项工作中，开发了玉米醇溶蛋白/壳聚糖纳米粒子（ZCPs-Q）来封装槲皮素，以克服其较低的水溶性和不稳定性，并同时增强其细胞摄取和细胞内抗氧化活性。该策略将槲皮素在水中和PBS（7.4）中的溶解度分别提高了753.6和9.95倍，并且封装在ZCP中的槲皮素在紫外线照射和热处理后保持稳定。ZCPs-Q可通过抑制ROS的产生显着减弱AAPH诱导的红细胞溶血。它可以将细胞内抗氧化酶（SOD和GSH-Px）的活性恢复到正常水平，并抑制细胞内丙二醛（MDA）的形成。同时，ZCPs-Q在具有EC _50的HepG2细胞中显示出强大的抗氧化活性值31.18μg/ mL，低于游离槲皮素的41.02μg/ mL。ZCPs增强了槲皮素在Caco-2细胞中的吸收效率，这有助于改善通过CAA分析和AAPH诱导的红细胞溶血分析评估的细胞抗氧化活性（CAA）。所设计的途径特别适合于水不溶性营养保健品的封装，并适于增强细胞摄取和CAA。

更新日期：2018-11-08

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