To guide barrier membrane choice in the treatment of peri-implant alveolar bone defects, we evaluated guided bone regeneration (GBR) using titanium (Ti) mesh or Bio-Gide membrane, independently or in combination, for repair of alveolar bone defects in Beagle dogs. Six months after extraction of the mandibular premolars and first molars from three beagle dogs, we inserted implants assigned into 3 groups and covered with the following membrane combinations: Group A: Implant + Bio-Oss + Ti-mesh, Group B: Implant + Bio-Oss + Bio-Gide, and Group C: Implant + Bio-Oss + Ti-mesh + Bio-Gide. At 6 months, micro-CT revealed that bone volume/total volume (BV/TV), trabecular number (Tb.N), and trabecular thickness (Tb.Th) was significantly greater in Group C than the other two groups, while trabecular separation (Tb.Sp) was significantly lower, suggesting improved bone regeneration. The distance between bands of three fluorescent tracking dyes was significantly greater in Group C, indicating faster deposition of new bone. The Bio-Oss particles were ideally integrated with newly deposited bone and bone thickness was significantly larger in Group C. These findings suggest that combination of Bio-Gide membrane and titanium mesh can effectively repair peri-implant alveolar bone defects, achieving enhanced bone regeneration compared to titanium mesh or Bio-Gide alone, and therefore providing a novel treatment concept for clinical implant surgery.

中文翻译：

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GBR修复比格犬植入物周围牙槽缺损的实验。

为了指导选择屏障膜来治疗植入物周围的牙槽骨缺损，我们评估了单独或组合使用钛（Ti）网或Bio-Gide膜的引导性骨再生（GBR）对Beagle犬的牙槽骨缺损的修复。从三只比格犬的下颌前磨牙和第一磨牙中取出六个月后，我们将植入物分为三组，并用以下膜组合覆盖：A组：植入物+ Bio-Oss + Ti-mesh，B组：植入物+ Bio -Oss + Bio-Gide，以及C组：植入物+ Bio-Oss + Ti-mesh + Bio-Gide。在6个月时，micro-CT显示，C组的骨体积/总体积（BV / TV），骨小梁数目（Tb.N）和骨小梁厚度（Tb.Th）显着大于其他两组，而骨小梁分离度（Tb.Sp）显着降低，提示骨骼再生得到改善。C组中三种荧光跟踪染料的条带之间的距离明显更大，表明新骨沉积更快。Bio-Oss颗粒理想地与新沉积的骨融合在一起，并且C组的骨厚度明显更大。这些发现表明，Bio-Gide膜和钛网的结合可以有效修复植入物周围的牙槽骨缺损，与之相比，骨再生得到增强单独使用钛网或Bio-Gide，因此为临床植入手术提供了一种新颖的治疗概念。