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Antimicrobial activity and action mechanism of triglycerol monolaurate on common foodborne pathogens
Food Control ( IF 5.6 ) Pub Date : 2019-04-01 , DOI: 10.1016/j.foodcont.2018.11.017
Song Zhang , Jian Xiong , Wenyong Lou , Zhengxiang Ning , Denghui Zhang , Jiguo Yang

Abstracts Triglycerol monolaurate (TGML) was widely used as an emulsifier in food, cosmetic and other products, and was generally accepted as safe. In the study, the inhibitory activity of TGML against common foodborne pathogens, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Listeria monocytogenes, Candida albicans and Cryptococcus neoformans was investigated with comparison to sodium benzoate and potassium sorbate. The minimum inhibitory concentrations (MIC) of TGML against six pathogens were 0.16, 0.16, 0.08, 0.04, 0.64 and 1.25 mg/mL, the minimum bactericidal concentrations (MBC) were 0.64, 1.25, 0.32, 0.16, 5.0 and 5.0 mg/mL, respectively, both lower than that of sodium benzoate and potassium sorbate. The MIC remained stable when the pH values increased from 3 to 9. Besides, the growth of Staphylococcus aureus and Listeria monocytogenes was almost inhibited for 72 h, whereas the inhibition effect on the rest four strains degraded at 36–48 h and 4–5 days, respectively. The action mechanism of TGML was further studied by detecting plasma membrane permeability, membrane surface damage and the impact of cell cycle and intracellular biomacromolecules synthesis. Results revealed that TGML increased cell membrane permeability, destroyed cell membrane integrity and caused depressions and holes, caused cell cycle arrest and biomacromolecules synthesis inhibition. These results suggest that TGML can effectively exert good inhibitory effect on bacteria and yeast by cell membrane action and intracellular inhibition.

中文翻译:

单月桂酸甘油三酯对常见食源性病原体的抗菌活性及作用机制

摘要 甘油单月桂酸酯 (TGML) 被广泛用作食品、化妆品和其他产品中的乳化剂,并被普遍认为是安全的。在该研究中,与苯甲酸钠和山梨酸钾相比,研究了 TGML 对常见食源性病原体、大肠杆菌、鼠伤寒沙门氏菌、金黄色葡萄球菌、单核细胞增生李斯特菌、白色念珠菌和新型隐球菌的抑制活性。TGML对六种病原体的最低抑菌浓度(MIC)分别为0.16、0.16、0.08、0.04、0.64和1.25 mg/mL,最低杀菌浓度(MBC)分别为0.64、1.25、0.32、0.16、5.0.0 mg/mL。分别低于苯甲酸钠和山梨酸钾。当 pH 值从 3 增加到 9 时,MIC 保持稳定。此外,金黄色葡萄球菌和单核细胞增生李斯特菌的生长在 72 小时内几乎被抑制,而对其余 4 株的抑制作用分别在 36-48 小时和 4-5 天下降。通过检测质膜通透性、膜表面损伤以及对细胞周期和细胞内生物大分子合成的影响,进一步研究了TGML的作用机制。结果表明,TGML增加细胞膜通透性,破坏细胞膜完整性并引起凹陷和孔洞,引起细胞周期停滞和生物大分子合成抑制。这些结果表明TGML可以通过细胞膜作用和细胞内抑制作用有效地对细菌和酵母发挥良好的抑制作用。而对其余四种菌株的抑制作用分别在 36-48 小时和 4-5 天降解。通过检测质膜通透性、膜表面损伤以及对细胞周期和细胞内生物大分子合成的影响,进一步研究了TGML的作用机制。结果表明,TGML增加细胞膜通透性,破坏细胞膜完整性并引起凹陷和孔洞,引起细胞周期停滞和生物大分子合成抑制。这些结果表明TGML可以通过细胞膜作用和细胞内抑制作用有效地对细菌和酵母发挥良好的抑制作用。而对其余四种菌株的抑制作用分别在 36-48 小时和 4-5 天降解。通过检测质膜通透性、膜表面损伤以及对细胞周期和细胞内生物大分子合成的影响,进一步研究了TGML的作用机制。结果表明,TGML增加细胞膜通透性,破坏细胞膜完整性并引起凹陷和孔洞,引起细胞周期停滞和生物大分子合成抑制。这些结果表明TGML可以通过细胞膜作用和细胞内抑制作用有效地对细菌和酵母发挥良好的抑制作用。膜表面损伤和细胞周期和细胞内生物大分子合成的影响。结果表明,TGML 增加细胞膜通透性,破坏细胞膜完整性并导致凹陷和孔洞,导致细胞周期停滞和生物大分子合成抑制。这些结果表明TGML可以通过细胞膜作用和细胞内抑制作用有效地对细菌和酵母发挥良好的抑制作用。膜表面损伤和细胞周期和细胞内生物大分子合成的影响。结果表明,TGML增加细胞膜通透性,破坏细胞膜完整性并引起凹陷和孔洞,引起细胞周期停滞和生物大分子合成抑制。这些结果表明TGML可以通过细胞膜作用和细胞内抑制作用有效地对细菌和酵母发挥良好的抑制作用。
更新日期:2019-04-01
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