活细胞表面电荷可视化,Journal of the American Chemical Society

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活细胞表面电荷可视化
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2016-02-25 , DOI: 10.1021/jacs.5b13153
David Perry ₁ , Binoy Paulose Nadappuram ₁ , Dmitry Momotenko ₁ , Philip D Voyias ₁ , Ashley Page ₁ , Gyanendra Tripathi ₁ , Bruno G Frenguelli ₁ , Patrick R Unwin ₁

Affiliation

扫描离子电导显微镜 (SICM) 被证明是一种用于活细胞定量纳米级表面电荷映射的强大技术。利用偏置调制 (BM) 方案，其中对填充电解质的纳米移液管中的准参考对电极 (QRCE) 与本体溶液中的 QRCE 之间的电位进行调制，结果表明，电池形貌和表面电荷可以在高空间分辨率下与动态电位测量同时测量细胞界面上的存在。通过探测细胞界面上的扩散双层 (DDL) 的特性来阐明表面电荷，并且该技术在低离子强度和典型生理（高离子强度）条件下都很敏感。将像素级自参考（校准）与强大的理论模型相结合的实验相结合，可以分析跨细胞界面的局部表面电荷变化，如在两个重要的生命系统上所证明的那样。首先，玉米根毛上的电荷映射表明，细胞尖端有很高的负表面电荷。其次，研究表明人类脂肪细胞表面存在明显的表面电荷分布，其作用是哺乳动物系统中脂质的储存和调节。这些是以前没有认识到的新特征，并且强调了它们对这些细胞功能的影响。正如在两个重要的生命系统上所证明的那样。首先，玉米根毛上的电荷映射表明，细胞尖端有很高的负表面电荷。其次，研究表明人类脂肪细胞表面存在明显的表面电荷分布，其作用是哺乳动物系统中脂质的储存和调节。这些是以前没有认识到的新特征，并且强调了它们对这些细胞功能的影响。正如在两个重要的生命系统上所证明的那样。首先，玉米根毛上的电荷映射表明，细胞尖端有很高的负表面电荷。其次，研究表明人类脂肪细胞表面存在明显的表面电荷分布，其作用是哺乳动物系统中脂质的储存和调节。这些是以前没有认识到的新特征，并且强调了它们对这些细胞功能的影响。

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Surface Charge Visualization at Viable Living Cells

Scanning ion conductance microscopy (SICM) is demonstrated to be a powerful technique for quantitative nanoscale surface charge mapping of living cells. Utilizing a bias modulated (BM) scheme, in which the potential between a quasi-reference counter electrode (QRCE) in an electrolyte-filled nanopipette and a QRCE in bulk solution is modulated, it is shown that both the cell topography and the surface charge present at cellular interfaces can be measured simultaneously at high spatial resolution with dynamic potential measurements. Surface charge is elucidated by probing the properties of the diffuse double layer (DDL) at the cellular interface, and the technique is sensitive at both low-ionic strength and under typical physiological (high-ionic strength) conditions. The combination of experiments that incorporate pixel-level self-referencing (calibration) with a robust theoretical model allows for the analysis of local surface charge variations across cellular interfaces, as demonstrated on two important living systems. First, charge mapping at Zea mays root hairs shows that there is a high negative surface charge at the tip of the cell. Second, it is shown that there are distinct surface charge distributions across the surface of human adipocyte cells, whose role is the storage and regulation of lipids in mammalian systems. These are new features, not previously recognized, and their implications for the functioning of these cells are highlighted.

更新日期：2016-02-25

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