The molybdenum disulfide (MoS₂) nanosheets functionalized fiber optic surface plasmon resonance (SPR) immunosensor has been reported for the sensitive detection of Escherichia coli (E. coli). The MoS₂ nanosheets were prepared by chemical exfoliation method. The synthesised nanostructures were characterized for their structural, morphological and optical properties. The E. coli monoclonal antibodies were successfully immobilized on the MoS₂ functionalized sensing platform via hydrophobic interactions. An alternative method simplifying the antibodies immobilization process by functionalization of 2D nanomaterial (MoS₂ nanosheets) for rapid (~15 mins) bacterial quantification is presented in this study. The immunosensor uses wavelength interrogation method and a strong linear relationship (R² = 0.994) was observed between spectral response of immunosensor and different concentration of E. coli. The nonspecificity and cross-reactivity studies of the developed immunosensor were investigated with detection of Salmonella Typhimurium and Staphylococcus aureus. To demonstrate the practical application, spiked samples of water and orange juice were analysed with acceptable recovery results. The label-free immunosensor exhibits better performance, detection limit (94 CFU/mL), high sensitivity (2.9 nm/1000 CFU mL⁻¹; 3135 nm/RIU) and profound specificity as compared to conventional fiber optic SPR sensor (detection limit: 391 CFU/mL, sensitivity: 0.6 nm/1000 CFU mL⁻¹; 1646 nm/RIU). This sensing platform shows promising applications in regular water and food quality monitoring for various pathogenic microorganisms.

据报道，二硫化钼（MoS ₂）纳米片功能化的光纤表面等离子体共振（SPR）免疫传感器可用于敏感检测大肠杆菌（E. coli）。通过化学剥落法制备MoS ₂纳米片。合成的纳米结构的结构，形态和光学特性进行了表征。的大肠杆菌单克隆抗体成功地固定在MOS上₂经由疏水相互作用官能感测平台。通过2D纳米材料（MoS ₂纳米片）用于快速（〜15分钟）细菌定量分析。该免疫传感器采用波长询问法，观察到免疫传感器的光谱响应与不同浓度的大肠杆菌之间存在很强的线性关系（R ² = 0.994）。通过检测鼠伤寒沙门氏菌和金黄色葡萄球菌，研究了开发的免疫传感器的非特异性和交叉反应性研究。为了证明实际应用，对加标的水和橙汁样品进行了分析，回收率令人满意。无标记免疫传感器具有更好的性能，检测限（94 CFU / mL），高灵敏度（2.9 nm / 1000 CFU mL ^-1）; 与传统的光纤SPR传感器相比具有3135 nm / RIU的高特异性（检测限：391 CFU / mL，灵敏度：0.6 nm / 1000 CFU mL ^-1； 1646 nm / RIU）。该传感平台在常规水和食品质量监测中对各种病原微生物显示出有希望的应用。