纳米粒子移位多个纳米孔的组行为。,Analytical Chemistry

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纳米粒子移位多个纳米孔的组行为。
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-10-29 00:00:00 , DOI: 10.1021/acs.analchem.8b03408
Chenyu Wen ₁ , Shuangshuang Zeng ₁ , Zhen Zhang ₁ , Shi-Li Zhang ₁

Affiliation

纳米孔已被实现为用于DNA测序，生物分子检查，化学分析，纳米颗粒检测等的纳米传感器。对于使用纳米孔阵列的高通量和并行测量，单个可寻址性已成为至关重要的技术解决方案，以便能够仔细检查每个孔处产生的信号。和每个纳米孔。在这里，通过检查移位多个纳米孔的纳米颗粒的团簇行为，研究了采用排列的纳米孔来执行传感器功能的替代途径。由于不需要单独的寻址能力，因此可以大大简化纳米孔器件以及微流控单元和读出电路的制造。实验上，显示少于10个孔的阵列能够分析具有良好信噪比的易位纳米颗粒。根据理论预测，如果可以更好地控制测量系统的噪声水平，则每个阵列有更多的孔（超过10个）可以执行高保真度分析。由于目标纳米颗粒的捕获截面较大，因此每个阵列上的孔更多，也可以在较低的浓度下进行更快的测量。通过实验改变孔的数量，纳米颗粒的浓度或施加在纳米孔上的偏压，我们已经确定了这种多事件过程的基本特征。通过表征易位过程中的平均孔隙电流和相关的标准偏差，并通过进行物理建模和广泛的数值模拟，我们已经显示出确定两种浓度超过4个数量级的易位纳米颗粒的大小和浓度的可能性。

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Group Behavior of Nanoparticles Translocating Multiple Nanopores

Nanopores have been implemented as nanosensors for DNA sequencing, biomolecule inspection, chemical analysis, nanoparticle detection, etc. For high-throughput and parallelized measurement using nanopore arrays, individual addressability has been a crucial technological solution in order to enable scrutiny of signals generated at each and every nanopore. Here, an alternative pathway of employing arrayed nanopores to perform sensor functions is investigated by examining the group behavior of nanoparticles translocating multiple nanopores. As no individual addressability is required, fabrication of nanopore devices along with microfluidic cells and readout circuits can be greatly simplified. Experimentally, arrays of less than 10 pores are shown to be capable of analyzing translocating nanoparticles with a good signal-to-noise margin. According to theoretical predictions, more pores (than 10) per array can perform high-fidelity analysis if the noise level of the measurement system can be better controlled. More pores per array would also allow for faster measurement at lower concentration because of larger capture cross sections for target nanoparticles. By experimentally varying the number of pores, the concentration of nanoparticles, or the applied bias voltage across the nanopores, we have identified the basic characteristics of this multievent process. By characterizing average pore current and associated standard deviation during translocation and by performing physical modeling and extensive numerical simulations, we have shown the possibility of determining the size and concentration of two kinds of translocating nanoparticles over 4 orders of magnitude in concentration. Hence, we have demonstrated the potential and versatility of the multiple-nanopore approach for high-throughput nanoparticle detection.

更新日期：2018-10-29

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