Chemiluminescence (CL) of luminol is a well-established technology for in vitro biochemical analysis of hydrogen peroxide (H₂O₂). However, applications of chemiluminescence as a signal transduction trigger for fluorophore release still remain to be exploited. In this paper, we rationally design a chemiluminescence-triggered fluorophore release approach for imaging of H₂O₂ in vivo. The o-benzyl alcohol-decorated block poly(carbonate)s copolymer, viz. PMPC−ONA, was synthesized affording the final formation of the H₂O₂ responsive micelles, in which luminol, fluorophore, and hemin were wrapped forming L/H/[email protected]−ONA nanoprobe. The presence of H₂O₂ diffuses into the nanoparticles and reacts with the hemin, generating high-energy reactive oxygen that could chemically excite the luminol triggering the chemiluminescence for cleaving nitrosobenzaldehyde recognition sites. The process could lead to destabilize the micelles and release the fluorescent indicator for imaging of H₂O₂. The concentration of H₂O₂ can be determined from 0.02 to 100 μM with a detection limit of 0.01 μM. The feasibility of L/H/[email protected]−ONA is demonstrated for investigating fluorescence imaging of endogenous H₂O₂ in cells and mice. As far as we know, this is the first paradigm of rationally designed chemiluminescence as a signal transformation trigger for fluorophore release.

鲁米诺的化学发光（CL）是一种用于过氧化氢（H ₂ O ₂）体外生化分析的成熟技术。然而，化学发光作为荧光团释放的信号转导触发的应用仍然有待开发。在本文中，我们合理设计了一种化学发光触发的荧光团释放方法，用于体内H ₂ O _2的成像。邻苯甲醇装饰的嵌段聚碳酸酯共聚物，即。合成了PMPC-ONA，提供了最终形成的H ₂ O ₂响应胶束，其中包裹了鲁米诺，荧光团和血红素，形成了L / H / [受电子邮件保护的] -ONA纳米探针。H的存在₂ O ₂扩散到纳米粒子中并与血红素反应，生成高能活性氧，该氧可以化学激发鲁米诺，触发化学发光，裂解亚硝基苯甲醛识别位点。该过程可能导致胶束不稳定并释放用于H ₂ O ₂成像的荧光指示剂。H ₂ O ₂的浓度可以在0.02至100μM之间确定，检出限为0.01μM。证明了L / H / [受电子邮件保护的] -ONA用于研究内源性H ₂ O _2的荧光成像的可行性在细胞和小鼠中。据我们所知，这是合理设计化学发光作为荧光团释放的信号转换触发的第一个范例。