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Protein Corona Formed from Different Blood Plasma Proteins Affects the Colloidal Stability of Nanoparticles Differently
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-10-22 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00743
Yan Teck Ho 1 , Nurul ‘Ain Azman 2 , Fion Wen Yee Loh 2 , Gabriella Kai Teng Ong 2 , Gokce Engudar 2 , Seth Allan Kriz 3 , James Chen Yong Kah 1, 2
Affiliation  

Significant progress in the characterization of protein corona has been made. However, insights on how the corona affects the aggregation of nanoparticles (NPs) and consequent biological identity are still lacking. Here, we examined how the corona formed from four major serum proteins, immunoglobulin G (IgG), fibrinogen (FBG), apolipoprotein A1 (ApoA1), and human serum albumin (HSA), over a range of concentrations affects the aggregation of gold NPs (AuNPs). We found that at physiological pH of 7.4, all four proteins aggregated the AuNPs at low concentrations but conferred colloidal stability at high concentrations due to the complete “corona coat” around individual AuNPs. Due to their immune-related functions, IgG and FBG aggregated the AuNPs to a greater extent compared to HSA and ApoA1 which were mostly involved in transport of small molecules. We then introduced the AuNP-corona formed from each protein into an acidic solution at pH 6.2 with high ionic concentration for up to 24 h as a model of the tumor microenvironment to examine for changes in their aggregation. We observed that protein corona formation sterically stabilized the AuNP-corona for all four proteins, resulting in a smaller increase in aggregation and size compared to citrate-capped AuNPs. This was especially true for corona formed at high protein:AuNP ratios. Our study therefore showed that the formation of a complete “corona coat” around NPs at sufficiently high protein:NP ratio was required for colloidal stability of designed NP systems in both physiological and cancer microenvironment to maintain efficiency and efficacy in cancer drug delivery.

中文翻译:

不同血浆蛋白形成的蛋白电晕对纳米颗粒的胶体稳定性影响不同

蛋白质电晕的表征已取得重大进展。但是,仍然缺乏关于电晕如何影响纳米颗粒(NP)聚集以及随之而来的生物学特性的见解。在这里,我们研究了在一定浓度范围内由四种主要血清蛋白(免疫球蛋白G(IgG),纤维蛋白原(FBG),载脂蛋白A1(ApoA1)和人血清白蛋白(HSA))形成的电晕如何影响金NP的聚集。 (AuNP)。我们发现,在7.4的生理pH值下,所有四种蛋白质在低浓度时都会聚集AuNP,但由于在各个AuNP周围形成完整的“电晕涂层”,因此在高浓度时会赋予胶体稳定性。由于与免疫相关的功能,与主要参与小分子转运的HSA和ApoA1相比,IgG和FBG聚集AuNP的程度更大。然后,我们将由每种蛋白质形成的AuNP电晕引入pH 6.2的高离子浓度酸性溶液中长达24小时,作为肿瘤微环境的模型,以检查其聚集的变化。我们观察到,蛋白质电晕的形成在空间上稳定了所有四种蛋白质的AuNP电晕,与柠檬酸盐封端的AuNPs相比,聚集和尺寸的增加较小。对于以高蛋白质:AuNP比形成的电晕尤其如此。因此,我们的研究表明,在生理和癌症微环境中,要在生理和癌症微环境中设计NP系统的胶体稳定性,就需要在蛋白质和NP比例足够高的NP周围形成完整的“电晕涂层”,以维持癌症药物递送的效率和功效。
更新日期:2018-10-22
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