Fats, oil and grease (FOG) are energy-dense wastes that substantially increase biomethane recovery. Shifts in the microbial community during anaerobic co-digestion of FOG was assessed to understand relationships between substrate digestion and microbial adaptations. Excessive addition of FOG inhibited the methanogenic activity during initial phase; however, it enhanced the ultimate methane production by 217% compared to the control. The dominance of Proteobacteria was decreased with a simultaneous increase in Firmicutes, Bacteriodetes, Synergistetes and Euryarchaeota during the co-digestion. A significant increase in Syntrophomonas (0.18–11%), Sporanaerobacter (0.14–6%) and Propionispira (0.02–19%) was observed during co-digestion, which substantiated their importance in acetogenesis. Among methanogenic Archaea, the dominance of Methanosaeta (94%) at the beginning of co-digestion was gradually replaced by Methanosarcina (0.52–95%). The absence/relatively low abundance of syntrophic acetate oxidizers and hydrogenotrophic methanogens, and dominance of acetoclastic methanogens suggested that methane generation during co-digestion of FOG was predominantly conducted through acetoclastic pathway led by Methanosarcina.

脂肪，油脂（FOG）是能量密集的废物，可大大提高生物甲烷的回收率。评估了FOG厌氧共消化过程中微生物群落的变化，以了解底物消化与微生物适应之间的关系。过量添加FOG会抑制初始阶段的产甲烷活性。然而，与对照相比，它使最终的甲烷产量增加了217％。在共消化过程中，变形杆菌的优势降低，同时硬菌，细菌杆菌，协同菌和真鳞菌同时增加。在甲显著增加Syntrophomonas（0.18-11％），Sporanaerobacter（0.14-6％）和Propionispira在共消化过程中观察到（0.02–19％），证实了它们在产乙酸中的重要性。中甲烷古，的显性Methanosaeta（94％）在共消化的开始通过逐渐代替甲烷（0.52-95％） 。不存在/相对低丰度的互养醋酸氧化剂和氢营养甲烷，和乙酸分解甲烷的显性的FOG的共消化主要通过由主导乙酸分解途径进行期间建议甲烷产生甲烷。