全基因组观点的面包小麦早期穗形成过程中的转录组动力学。,Scientific Reports

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全基因组观点的面包小麦早期穗形成过程中的转录组动力学。
Scientific Reports ( IF 3.8 ) Pub Date : 2018-10-18 , DOI: 10.1038/s41598-018-33718-y
Yongpeng Li ₁ , Xing Fu ₂ , Meicheng Zhao ₁ , Wei Zhang ₁ , Bo Li _{1,

3} , Diaoguo An ₄ , Junming Li ₁ , Aimin Zhang ₅ , Renyi Liu ₆ , Xigang Liu ₁

Affiliation

小麦穗发育是细胞增殖和分化的协调过程，具有独特的阶段和结构变化。但是，在此过程中基因表达的动态改变仍然是个谜。在这里，我们表征和解剖面包小麦穗分为六个发育阶段，并使用全基因组基因表达谱，以调查潜在的调控机制。任何两个给定阶段之间的高基因表达相关性表明，小麦早期穗增长受一小部分基因控制。在整个过程中，生长素信号传导增强，而细胞分裂素信号传导减少。此外，许多与应激反应相关的基因在双脊阶段高表达。在差异表达基因（DEG）中，鉴定出375个转录因子（TF）基因，其中水稻或拟南芥中的一些同源物被提议在分生组织维持，开花时间，分生组织起始或转变，花器官发育或对压力的反应中起作用。基因表达谱分析表明这些基因在小麦中具有相似或不同的表达模式。在早期峰值中表达了几种调控峰值发育的基因，其中发现了Earnesss per 3（Eps-3）可能在小穗分生组织的启动中起作用。我们的研究有助于发现与小麦顶端分生组织和发育相关的重要基因。基因表达谱分析表明这些基因在小麦中具有相似或不同的表达模式。在早期峰值中表达了几种调控峰值发育的基因，其中发现了Earnesss perse 3（Eps-3）可能在小穗分生组织的启动中起作用。我们的研究有助于发现与小麦顶端分生组织和发育相关的重要基因。基因表达谱分析表明这些基因在小麦中具有相似或不同的表达模式。在早期峰值中表达了几种调控峰值发育的基因，其中发现了Earnesss per 3（Eps-3）可能在小穗分生组织的启动中起作用。我们的研究有助于发现与小麦顶端分生组织和发育相关的重要基因。

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A Genome-wide View of Transcriptome Dynamics During Early Spike Development in Bread Wheat.

Wheat spike development is a coordinated process of cell proliferation and differentiation with distinctive phases and architecture changes. However, the dynamic alteration of gene expression in this process remains enigmatic. Here, we characterized and dissected bread wheat spike into six developmental stages, and used genome-wide gene expression profiling, to investigate the underlying regulatory mechanisms. High gene expression correlations between any two given stages indicated that wheat early spike development is controlled by a small subset of genes. Throughout, auxin signaling increased, while cytokinin signaling decreased. Besides, many genes associated with stress responses highly expressed during the double ridge stage. Among the differentially expressed genes (DEGs), were identified 375 transcription factor (TF) genes, of which some homologs in rice or Arabidopsis are proposed to function in meristem maintenance, flowering time, meristem initiation or transition, floral organ development or response to stress. Gene expression profiling demonstrated that these genes had either similar or distinct expression pattern in wheat. Several genes regulating spike development were expressed in the early spike, of which Earliness per se 3 (Eps-3) was found might function in the initiation of spikelet meristem. Our study helps uncover important genes associated with apical meristem morphology and development in wheat.

更新日期：2018-10-19

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