Biofilm‐Inspired Encapsulation of Probiotics for the Treatment of Complex Infections,Advanced Materials

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Biofilm‐Inspired Encapsulation of Probiotics for the Treatment of Complex Infections
Advanced Materials ( IF 27.4 ) Pub Date : 2018-10-17 , DOI: 10.1002/adma.201803925
Zhihao Li ₁ , Adam M. Behrens ₁ , Nitzan Ginat ₁ , Stephany Y. Tzeng ₁ , Xueguang Lu ₁ , Sarit Sivan ₂ , Robert Langer ₁ , Ana Jaklenec ₁

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The emergence of antimicrobial resistance poses a major challenge to healthcare. Probiotics offer a potential alternative treatment method but are often incompatible with antibiotics themselves, diminishing their overall therapeutic utility. This work uses biofilm‐inspired encapsulation of probiotics to confer temporary antibiotic protection and to enable the coadministration of probiotics and antibiotics. Probiotics are encapsulated within alginate, a crucial component of pseudomonas biofilms, based on a simple two‐step alginate cross‐linking procedure. Following exposure to the antibiotic tobramycin, the growth and metabolic activity of encapsulated probiotics are unaffected by tobramycin, and they show a four‐log survival advantage over free probiotics. This results from tobramycin sequestration on the periphery of alginate beads which prevents its diffusion into the core but yet allows probiotic byproducts to diffuse outward. It is demonstrated that this approach using tobramycin combined with encapsulated probiotic has the ability to completely eradicate methicillin‐resistant Staphylococcus aureus and Pseudomonas aeruginosa in coculture, the two most widely implicated bacteria in chronic wounds.

中文翻译：

生物膜启发的益生菌包囊剂用于复杂感染的治疗

抗菌素耐药性的出现对医疗保健构成了重大挑战。益生菌提供了一种潜在的替代治疗方法，但通常与抗生素本身不相容，从而降低了它们的整体治疗效用。这项工作采用了受生物膜启发的益生菌包封技术，可以暂时保护抗生素并实现益生菌和抗生素的共同给药。益生菌根据简单的两步藻酸盐交联程序，被封装在藻酸盐中，藻酸盐是假单胞菌生物膜的重要组成部分。暴露于抗生素妥布霉素后，封装的益生菌的生长和代谢活性不受妥布霉素的影响，与游离益生菌相比，它们具有四对数的生存优势。这是由于妥布霉素螯合在藻酸盐珠的外围而造成的，这阻止了藻酸盐珠扩散到核心中，但允许益生菌副产物向外扩散。证明了这种方法与妥布霉素联合包封的益生菌结合使用具有完全消除耐甲氧西林的能力共培养中的金黄色葡萄球菌和铜绿假单胞菌是慢性伤口中影响最广的两种细菌。

更新日期：2018-10-17

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