A first-of-a-kind latent electrochemical redox probe, ferrocene carbamate phenyl acrylate (FCPA), was developed for the selective detection of cysteine (Cys) and aminoacylase (ACY-1). The electrochemical signal generated by this probe was shown to be highly specific to Cys and insensitive to other amino acids and biological redox reactants. The FCPA-incorporated electrochemical sensor exhibited a broad dynamic range of 0.25–100 μM toward Cys. This probe also proficiently monitored the ACY-1-catalyzed biochemical transformation of N-acetylcysteine (NAC) into Cys, and this proficiency was used to develop an electrochemical assay for quantifying active ACY-1, which it did so in a dynamic range of 10–200 pM (0.1–2 mU/cm³) with a detection limit of 1 pM (0.01 mU/cm³). Furthermore, the probe was utilized in real-time tracking and quantification of cellular Cys production, specifically in Escherichia coli W3110, along with a whole blood assay to determine levels of Cys and spiked ACY-1 in blood with a reliable analytical performance.

中文翻译：

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电化学分子开关用于在活细胞和全血中对半胱氨酸进行选择性分析以及对氨酰酶-1的定量

开发了第一种潜在的电化学氧化还原探针，即二茂铁氨基甲酸酯丙烯酸苯酯（FCPA），用于选择性检测半胱氨酸（Cys）和氨酰基酶（ACY-1）。该探针产生的电化学信号显示出对Cys高度特异性，对其他氨基酸和生物氧化还原反应物不敏感。装有FCPA的电化学传感器对Cys表现出0.25–100μM的宽动态范围。该探针还可以有效地监测ACY-1催化的N-乙酰半胱氨酸（NAC）向Cys的生化转化，并且该水平用于开发用于定量活性ACY-1的电化学分析，该分析在10的动态范围内进行–200 pM（0.1–2 mU / cm ³），检出限为1 pM（0.01 mU / cm ^3））。此外，该探针还用于实时跟踪和定量测定细胞中Cys的产量，特别是在大肠杆菌W3110中，还可以通过全血测定法来测定血液中Cys的水平和加标的ACY-1，并具有可靠的分析性能。