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Generation of two human isogenic iPSC lines from fetal dermal fibroblasts
Stem Cell Research ( IF 0.8 ) Pub Date : 2018-10-12 , DOI: 10.1016/j.scr.2018.10.004
Rashmi Tandon , Björn Brändl , Natalya Baryshnikova , Alexandro Landshammer , Laura Steenpaß , Oliver Keminer , Ole Pless , Franz-Josef Müller

Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. Previously, the same fetal fibroblasts were reprogrammed multiple times in a study comparing other reprogramming methods. As a consequence, the genomes have been sequenced multiple times. Both new cell lines offer the opportunity to study basic stem cell biology and model human disease. They can be applied as reference cell lines for creating isogenic clones bearing disease mutations on a well-characterized genomic background, as both cell lines have demonstrated excellent differentiation capacity in multiple labs.

Resource table

Unique stem cell lines identifierZIPi013-B
ZIPi013-E
Alternative names of stem cell linesZIP13K2 (ZIPi013-B)
ZIP13K5 (ZIPi013-E)
InstitutionZentrum für Integrative Psychiatrie gGmbH, Kiel, Germany
Contact information of distributorPD Dr. Franz-Josef Müller, [email protected]
Type of cell linesiPSC
Originhuman
Cell SourceFibroblasts
ClonalityClonal
Method of reprogrammingTransgene free, episomal
Multiline rationaleIsogenic clones
Gene modificationNO
Type of modificationN/A
Associated diseaseN/A
Gene/locusN/A
Method of modificationN/A
Name of transgene or resistanceN/A
Inducible/constitutive systemN/A
Date archived/stock dateStock date ZIPi013-B 8th December 2017, stock date ZIPi013-E 12th December 2017
Cell line repository/bankN/A
Ethical approvalhttps://www.sciencellonline.com/technical-support/ethical-statement.html
Ethikkommission der medizinischen Fakultät der Christian-Albrechts-Universität zu Kiel, approval number A145/11



中文翻译:

从胎儿真皮成纤维细胞生成两条人类同基因iPSC系

通过使用游离型载体对胎儿真皮成纤维细胞进行重编程,生成了两个同基因的hiPSC系ZIPi013-B和ZIPi013-E。以前,同一项胎儿成纤维细胞在一项比较其他重编程方法的研究中被重编程了多次。结果,基因组已被多次测序。两种新的细胞系都提供了研究基本干细胞生物学和模拟人类疾病的机会。它们可作为参考细胞系,用于在基因组充分表征的背景上创建携带疾病突变的同基因克隆,因为两种细胞系均已在多个实验室中证明具有出色的分化能力。

资源表

唯一的干细胞系标识符邮编013-B
邮编013-E
干细胞系的替代名称ZIP13K2(ZIPi013-B)
ZIP13K5(ZIPi013-E)
机构Zentrumfür综合精神病学有限公司,德国基尔
经销商联系方式PD Franz-JosefMüller博士,[受电子邮件保护]
细胞系类型互联网服务中心
起源人类
细胞来源成纤维细胞
克隆性无性系
重新编程的方法无转基因,游离型
多行原理同基因克隆
基因修饰
修改类型不适用
相关疾病不适用
基因/基因座不适用
修改方法不适用
转基因或抗性的名称不适用
诱导/本构系统不适用
存档日期/库存日期库存日期ZIPi013-B 2017年12月8日,库存日期ZIPi013-E 2017年12月12日
细胞系库/库不适用
道德认可https://www.sciencellonline.com/technical-support/ethical-statement.html
德意志基督教大学-基辅基督教大学,批准号A145 / 11

更新日期:2018-10-12
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