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Comparative transcriptomic analysis reveals common molecular factors responsive to heat and drought stress in Agrostis stolonifera.
Scientific Reports ( IF 3.8 ) Pub Date : 2018-Oct-12 , DOI: 10.1038/s41598-018-33597-3 Yi Xu 1 , Bingru Huang 1
Scientific Reports ( IF 3.8 ) Pub Date : 2018-Oct-12 , DOI: 10.1038/s41598-018-33597-3 Yi Xu 1 , Bingru Huang 1
Affiliation
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Heat and drought stress are primary abiotic stresses confining growth of cool-season grass species during summer. The objective of this study was to identify common molecular factors and metabolic pathways associated with heat and drought responses in creeping bentgrass (Agrostis stolonifera) by comparative analysis of transcriptomic profiles between plants exposed to heat and drought stress. Plants were exposed to heat stress (35/30 °C day/night temperature) or drought stress by withholding irrigation for 21 d in growth chambers. Transcriptomic profiling by RNA-seq in A. stolonifera (cv. 'Penncross') found 670 commonly up-regulated and 812 commonly down-regulated genes by heat and drought stress. Transcriptional up-regulations of differentially expressed genes (DEGs) due to heat and drought stress include genes that were highly enriched in oxylipin biosynthetic process and proline biosynthetic process. Transcriptional down-regulations of genes under heat and drought stress were highly enriched and involved in thiamine metabolic process and calcium sensing receptor. These commonly-regulated genes by heat and drought stress identified in A. stolonifera suggested that drought and heat responses shared such common molecular factors and pathways, which could be potential candidate genes for genetic modification of improving plant tolerance to the combined heat and drought stress.
中文翻译：
比较转录组分析揭示了剪股草响应热和干旱胁迫的常见分子因子。
热和干旱胁迫是限制夏季冷季草种生长的主要非生物胁迫。本研究的目的是通过对暴露于热和干旱胁迫的植物之间的转录组谱进行比较分析，确定与匍匐翦股颖（Agrostis stolonifera）热和干旱反应相关的常见分子因子和代谢途径。通过在生长室中停止灌溉 21 天，将植物暴露于热应激（35/30°C 白天/夜间温度）或干旱应激。通过 RNA-seq 对 A. stolonifera（cv. 'Penncross'）进行转录组分析发现，热和干旱胁迫下有 670 个普遍上调基因和 812 个普遍下调基因。由于热和干旱胁迫引起的差异表达基因（DEG）的转录上调包括在氧脂素生物合成过程和脯氨酸生物合成过程中高度富集的基因。热和干旱胁迫下基因的转录下调高度富集，并参与硫胺素代谢过程和钙敏感受体。在匍匐花中鉴定出的这些受热和干旱胁迫共同调控的基因表明，干旱和热反应具有如此共同的分子因子和途径，这可能是提高植物对热和干旱联合胁迫耐受性的遗传修饰的潜在候选基因。
更新日期：2018-10-12
中文翻译：
比较转录组分析揭示了剪股草响应热和干旱胁迫的常见分子因子。
热和干旱胁迫是限制夏季冷季草种生长的主要非生物胁迫。本研究的目的是通过对暴露于热和干旱胁迫的植物之间的转录组谱进行比较分析，确定与匍匐翦股颖（Agrostis stolonifera）热和干旱反应相关的常见分子因子和代谢途径。通过在生长室中停止灌溉 21 天，将植物暴露于热应激（35/30°C 白天/夜间温度）或干旱应激。通过 RNA-seq 对 A. stolonifera（cv. 'Penncross'）进行转录组分析发现，热和干旱胁迫下有 670 个普遍上调基因和 812 个普遍下调基因。由于热和干旱胁迫引起的差异表达基因（DEG）的转录上调包括在氧脂素生物合成过程和脯氨酸生物合成过程中高度富集的基因。热和干旱胁迫下基因的转录下调高度富集，并参与硫胺素代谢过程和钙敏感受体。在匍匐花中鉴定出的这些受热和干旱胁迫共同调控的基因表明，干旱和热反应具有如此共同的分子因子和途径，这可能是提高植物对热和干旱联合胁迫耐受性的遗传修饰的潜在候选基因。
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