Corneal disease is a major cause of blindness and keratoplasty is an effective treatment method. A freeze-dried collagen film (FD-Col) with micro-rough surface structure for corneal epithelial repair was reported in our previous studies. In this research, we conducted a more comprehensive study on the FD-Col film. Besides with excellent mechanical property and optical transparency, the FD-Col film also has good penetrating ability in nutrient solutions. The permeability coefficient of the FD-Col in NaCl and tryptophan solution is (2.58 ± 0.47) × 10 cm/s and (2.67 ± 0.13) × 10 cm/s, respectively. In addition, the morphology change of the FD-Col film before and after water absorption is relatively stable suggesting that this film can be fabricated with various dimensions easily. Moreover, corneal lamellar keratoplasty shows that the FD-Col film can be sutured in rabbit's ocular surface and the re-epithelization process in vivo is complete in about 12 days, and the transparency is restored quickly in the first month. Corneal rejection reaction, neovascularization and keratoconus are not observed within 2 months. This FD-Col film, which can be prepared in large quantities and at low cost, should have potential application in corneal repair in the future.

中文翻译：

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具有微粗糙表面的胶原蛋白膜可以促进角膜上皮化过程，从而实现角膜修复


角膜疾病是导致失明的主要原因，角膜移植术是有效的治疗方法。我们之前的研究报道了一种具有微粗糙表面结构的用于角膜上皮修复的冻干胶原膜（FD-Col）。在本研究中，我们对FD-Col薄膜进行了更全面的研究。 FD-Col薄膜除了具有优异的机械性能和光学透明性外，在营养液中还具有良好的渗透能力。 FD-Col在氯化钠和色氨酸溶液中的渗透系数分别为（2.58±0.47）×10 cm/s和（2.67±0.13）×10 cm/s。此外，FD-Col薄膜吸水前后的形貌变化相对稳定，表明该薄膜可以容易地制备成各种尺寸。而且，角膜板层角膜移植术显示，FD-Col膜可以缝合在兔眼表，体内的再上皮过程在12天左右完成，第一个月就很快恢复透明度。 2个月内未观察到角膜排斥反应、新生血管和圆锥角膜。这种FD-Col薄膜可以大批量、低成本制备，未来在角膜修复方面应该有潜在的应用。