功能性核酸探针可同时监测生物体中的K <sup>+</sup>和原卟啉IX,Analytical Chemistry

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功能性核酸探针可同时监测生物体中的K ⁺和原卟啉IX
Analytical Chemistry ( IF 6.7 ) Pub Date : 2016-02-11 00:00:00 , DOI: 10.1021/acs.analchem.5b04936
Hanjun Cheng _{1,

2} , Xuefeng Qiu ₃ , Xiaozhi Zhao ₃ , Wei Meng ₄ , Da Huo ₁ , Hui Wei ₁

Affiliation

并行监测K ⁺和原卟啉IX（PPIX）至关重要，因为它们不仅参与多种生物学过程，而且在许多细胞途径中彼此紧密联系。但是，目前尚不存在可以满足在活生物体中并行和体内检测K ⁺和PPIX的要求的方法。在本文中，我们展示了一种基于功能核酸（FNA）的技术，用于并行监测活体动物中的K ⁺和PPIX。具体而言，选择的富含G的FNA探针被K ⁺选择性诱导形成平行的G-四链体。然后，平行的G-四链体显着增强了PPIX的荧光。因此，通过用G-四链体和K ⁺ / PPIX调节荧光“开启”，可以检测到K ⁺和PPIX。在验证开发的用于选择性和灵敏检测K ^{+的方法后}和PPIX体外，同时监测了它们在各种生理和病理过程后在活生物体（即活脑和肿瘤）中的动态变化。当前的研究不仅为检测金属离子和生物活性分子提供了一种通用方法，而且为研究其在调节各种生物过程中的协同功能提供了一种方法。它还可能有助于改善PPIX和5-ALA的成像和治疗效果。

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Functional Nucleic Acid Probe for Parallel Monitoring K+ and Protoporphyrin IX in Living Organisms

Parallel monitoring of K⁺ and protoporphyrin IX (PPIX) is of vital importance because they are not only involved in a variety of biological processes but also closely linked to each other in numerous cellular pathways. However, there are currently no existing methods that can meet the requirements for parallel and in vivo detection of K⁺ and PPIX in living organisms. Herein, we demonstrated a functional nucleic acid (FNA)-based technique for parallel monitoring of K⁺ and PPIX in living animals. Specifically, the selected G-rich FNA probe was selectively induced to form a parallel G-quadruplex by K⁺. The parallel G-quadruplex then remarkably enhanced the fluorescence of PPIX. Thus, by modulating the fluorescence “turn on” with the G-quadruplex and K⁺/PPIX, both K⁺ and PPIX could be detected. After validating the developed method for selective and sensitive detection of K⁺ and PPIX in vitro, their dynamic changes in living organisms (i.e., living brains and tumors) following various physiological and pathological processes were simultaneously monitored. The current study not only provides a general method for the detection of metal ions and bioactive molecules but also presents a way to investigate their synergistic functions in the regulation of various biological processes. It may also be helpful for improving the imaging and therapeutic efficacy of PPIX and 5-ALA.

更新日期：2016-02-11

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