Spatial comprehensive two-dimensional liquid chromatography (^xLC×^xLC) may be an efficient approach to achieve high peak capacities in relatively short analysis times, thanks to parallel second-dimension separations [1,2]. A key issue to reach the potential of ^xLC×^xLC is to achieve adequate flow control and confinement of the analytes to the desired regions, i.e. confinement in the first-dimension direction and subsequently homogeneous flow in the second dimension. To achieve these goals we propose the TWIST concept (TWo-dimensional Insertable Separation Tool), a modular device that includes an internal first-dimension (¹D) part that is cylindrical and rotatable. This internal part features a series of through-holes, each of which is perpendicular to the direction of the ¹D flow. The internal part is inserted in the cylindrical casing of the external part. The internal diameter of the casing is marginally larger than the external diameter of the internal part. The external part also comprises a flow distributor and second-dimension (²D) channels. During the ¹D injection and development, the channel is placed in a position where the through-holes are facing the wall of the external part, such that the liquid remains confined within the ¹D channel. Thereafter, to realize the transfer to the second dimension (²D injection), the ¹D channel is rotated, so that the holes of the internal part are aligned with the holes on the external part, allowing a transversal flow of the ²D mobile phase from the distributor through the ¹D channel and eventually into the ²D area.

由于平行的二维分离[1,2]，空间综合二维液相色谱（^x LC ^{x x} LC）可能是在相对较短的分析时间内实现高峰容量的有效方法。达到^x LC× ^x LC潜力的关键问题是实现足够的流量控制并将分析物限制在所需区域内，即在第一维方向上限制，然后在第二维方向上均匀流动。为了实现这些目标，我们提出的概念TWIST（二维可插入分离工具），模块化装置，其包括一个内部第一维（¹D）圆柱形且可旋转的零件。这个内部部分设有一系列通孔，其中的每一个垂直于所述方向的¹ d流动。内部部分插入外部部分的圆柱形外壳中。壳体的内径略大于内部的外径。外部还包括流量分配器和第二维（² D）通道。在¹ d注射和发展中，信道被放置在其中的通孔面向外部部分的壁的位置，使得液体遗体内局限于¹ d通道。之后，实现向第二维（²D注入），旋转¹ D通道，使内部部件的孔与外部部件上的孔对齐，从而使² D流动相从分配器通过¹ D通道横向流动，并最终流入的² d的区域。