Pretreatment of lignocellulosic biomass results in the formation of byproducts (furfural, hydroxymethylfurfural [HMF], vanillin, acetate etc.), which affect microbial growth and productivity. Furfural (0.02%), HMF (0.04%), and acetate (0.6%) showed positive effects on Ralstonia eutropha 5119 growth and polyhydroxyalkanoate (PHA) production, while vanillin exhibited negative effects. Response optimization and interaction studies between the variables glucose, ammonium chloride, furfural, HMF, and acetate using the response surface methodology resulted in maximum PHA production (2.1 g/L) at optimal variable values of 15.3 g/L, 0.43 g/L, 0.04 g/L, 0.05 g/L, and 2.34 g/L, respectively. Different lignocellulosic biomass hydrolysates (LBHs), including barley biomass hydrolysate (BBH), Miscanthus biomass hydrolysate (MBH), and pine biomass hydrolysate (PBH), were evaluated as potential carbon sources for R. eutropha 5119 and resulted in 1.8, 2.0, and 1.7 g/L PHA production, respectively. MBH proved the best carbon source, resulted in higher biomass (Y_x/s, 0.31 g/g) and PHA (Y_p/s, 0.14 g/g) yield.

木质纤维素生物质的预处理导致副产物（糠醛，羟甲基糠醛[HMF]，香兰素，乙酸盐等）的形成，这些副产物影响微生物的生长和生产力。糠醛（0.02％），HMF（0.04％）和乙酸盐（0.6％）对富营养小球藻（Ralstonia eutropha 5119）的生长和多羟基链烷酸酯（PHA）的产生表现出积极的影响，而香兰素则表现出负面的影响。使用响应面方法进行响应优化和变量葡萄糖，氯化铵，糠醛，HMF和乙酸盐之间的相互作用研究，可在最佳变量值为15.3 g / L，0.43 g / L的情况下，最大PHA产量（2.1 g / L），分别为0.04 g / L，0.05 g / L和2.34 g / L。不同的木质纤维素生物质水解产物（LBHs），包括大麦生物质水解产物（BBH），芒草生物质水解物（MBH）和松树生物质水解物（PBH）被评估为富营养罗汉果5119的潜在碳源，分别产生了1.8 g / L，2.0 g / L和1.7 g / L的PHA。MBH被证明是最佳的碳源，可产生更高的生物量（Y _{x / s，} 0.31 g / g）和PHA（Y _{p / s，} 0.14 g / g）。