植物病毒纳米粒子的蛋白质电晕会影响其分散特性，细胞相互作用和体内命运,Small

当前位置： X-MOL 学术 › Small › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

植物病毒纳米粒子的蛋白质电晕会影响其分散特性，细胞相互作用和体内命运
Small ( IF 13.0 ) Pub Date : 2016-02-08 , DOI: 10.1002/smll.201502458
Andrzej S. Pitek ₁ , Amy M. Wen ₁ , Sourabh Shukla ₁ , Nicole F. Steinmetz _{1,

2,

3,

4,

5}

Affiliation

诸如血浆之类的体液中的生物分子会吸附到纳米颗粒的表面并影响其生物学特性。这种现象称为蛋白质电晕，在合成纳米技术领域已得到充分确立，但尚未在植物病毒纳米粒子（VNP）的背景下进行描述。烟草花叶病毒衍生的VNP之间的相互作用（TMV）和血浆蛋白进行了研究，发现与合成颗粒的电晕相比，VNP蛋白的电晕明显不足。形成的电晕主要是补体蛋白和免疫球蛋白，它们的结合可以通过聚乙二醇化VNP表面来减少。在癌症和血栓形成的情况下，研究了VNP蛋白电晕对分子识别和细胞靶向的影响。含有聚乙二醇（PEG）和针对整联蛋白或血纤蛋白（原）的肽配体的功能化TMV棒库显示出不同的分散特性，细胞相互作用和体内命运，具体取决于蛋白质电晕的性质，影响靶标特异性和非巨噬细胞的特定清除作用。

"点击查看英文标题和摘要"

The Protein Corona of Plant Virus Nanoparticles Influences their Dispersion Properties, Cellular Interactions, and In Vivo Fates

Biomolecules in bodily fluids such as plasma can adsorb to the surface of nanoparticles and influence their biological properties. This phenomenon, known as the protein corona, is well established in the field of synthetic nanotechnology but has not been described in the context of plant virus nanoparticles (VNPs). The interaction between VNPs derived from Tobacco mosaic virus (TMV) and plasma proteins is investigated, and it is found that the VNP protein corona is significantly less abundant compared to the corona of synthetic particles. The formed corona is dominated by complement proteins and immunoglobulins, the binding of which can be reduced by PEGylating the VNP surface. The impact of the VNP protein corona on molecular recognition and cell targeting in the context of cancer and thrombosis is investigated. A library of functionalized TMV rods with polyethylene glycol (PEG) and peptide ligands targeting integrins or fibrin(ogen) show different dispersion properties, cellular interactions, and in vivo fates depending on the properties of the protein corona, influencing target specificity, and non‐specific scavenging by macrophages. Our results provide insight into the in vivo properties of VNPs and suggest that the protein corona effect should be considered during the development of efficacious, targeted VNP formulations.

更新日期：2016-02-08

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南