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A Colorimetric Sensor for the Visual Detection of Azodicarbonamide in Flour Based on Azodicarbonamide-Induced Anti-Aggregation of Gold Nanoparticles
ACS Sensors ( IF 8.2 ) Pub Date : 2018-09-21 00:00:00 , DOI: 10.1021/acssensors.8b00705 Zhiqiang Chen 1 , Lian Chen 1 , Ling Lin 1 , Yongning Wu 2 , FengFu Fu 1
ACS Sensors ( IF 8.2 ) Pub Date : 2018-09-21 00:00:00 , DOI: 10.1021/acssensors.8b00705 Zhiqiang Chen 1 , Lian Chen 1 , Ling Lin 1 , Yongning Wu 2 , FengFu Fu 1
Affiliation
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Azodicarbonamide (ADA) in flour products can be converted into carcinogenic biurea and semicarbazide hydrochloride after baking. Thus, it is mandatory to determine ADA in flour. We herein developed a colorimetric method for the rapid and visual detection of ADA in flour based on glutathione (GSH)-induced gold nanoparticles (AuNPs) aggregation and specific reaction between ADA and GSH. The GSH can react to AuNPs via Au-SH covalent bond to form a network structure, which leads to AuNPs aggregation to produce color change, whereas ADA can specifically react with GSH to lead to the coupling of two GSH molecules, which makes GSH lose a −SH group and thus decreases the aggregation degree of AuNPs induced by GSH. This provided a platform for field-portable colorimetric detection of ADA. The colorimetric sensor can be used to detect as little as 0.33 μM (38.3 ppb) of ADA by naked eye observation and 0.23 μM (26.7 ppb) of ADA by spectrophotometry within 2 h. The method was successfully used to detect ADA in flour with a recovery of 91–104% and a relative standard deviation (RSD) < 6%. The visual detection limit of sensor is lower than the ADA limitation in flour (45 mg/kg), which makes the sensor a potential approach for the instrument-free visual and on-site detection of ADA in flour.
中文翻译：
基于偶氮二甲酰胺诱导的金纳米粒子抗聚集的比色传感器，用于视觉检测面粉中的偶氮二甲酰胺。
面粉产品中的偶氮二甲酰胺（ADA）可以在烘烤后转化为致癌的双脲和盐酸氨基脲。因此，必须测定面粉中的ADA。我们在本文中开发了一种基于谷胱甘肽（GSH）诱导的金纳米颗粒（AuNPs）聚集和ADA与GSH之间特异性反应的面粉中ADA快速可见检测的比色方法。GSH可以通过Au-SH共价键与AuNPs反应形成网络结构，从而导致AuNPs聚集以产生颜色变化，而ADA可以与GSH特异性反应以导致两个GSH分子偶联，从而使GSH失去一个-SH组，因此降低了GSH诱导的AuNPs的聚集度。这为ADA的现场便携式比色检测提供了一个平台。比色传感器可用于检测低至0的值。肉眼观察到33μM（38.3 ppb）的ADA，分光光度法在2小时内获得0.23μM（26.7 ppb）的ADA。该方法已成功用于面粉中ADA的检测，回收率为91–104％，相对标准偏差（RSD）<6％。传感器的视觉检测极限低于面粉中的ADA限制（45 mg / kg），这使该传感器成为无仪器视觉和现场检测面粉中ADA的潜在方法。
更新日期：2018-09-21
中文翻译：
基于偶氮二甲酰胺诱导的金纳米粒子抗聚集的比色传感器，用于视觉检测面粉中的偶氮二甲酰胺。
面粉产品中的偶氮二甲酰胺（ADA）可以在烘烤后转化为致癌的双脲和盐酸氨基脲。因此，必须测定面粉中的ADA。我们在本文中开发了一种基于谷胱甘肽（GSH）诱导的金纳米颗粒（AuNPs）聚集和ADA与GSH之间特异性反应的面粉中ADA快速可见检测的比色方法。GSH可以通过Au-SH共价键与AuNPs反应形成网络结构，从而导致AuNPs聚集以产生颜色变化，而ADA可以与GSH特异性反应以导致两个GSH分子偶联，从而使GSH失去一个-SH组，因此降低了GSH诱导的AuNPs的聚集度。这为ADA的现场便携式比色检测提供了一个平台。比色传感器可用于检测低至0的值。肉眼观察到33μM（38.3 ppb）的ADA，分光光度法在2小时内获得0.23μM（26.7 ppb）的ADA。该方法已成功用于面粉中ADA的检测，回收率为91–104％，相对标准偏差（RSD）<6％。传感器的视觉检测极限低于面粉中的ADA限制（45 mg / kg），这使该传感器成为无仪器视觉和现场检测面粉中ADA的潜在方法。
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