当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Improved Method for Isolation and Purification of Underivatized Amino Acids for Radiocarbon Analysis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-09-16 00:00:00 , DOI: 10.1021/acs.analchem.8b02693 Naoto F. Ishikawa 1, 2 , Yu Itahashi 2, 3 , Thomas M. Blattmann 1 , Yoshinori Takano 2 , Nanako O. Ogawa 2 , Masako Yamane 2, 4, 5 , Yusuke Yokoyama 4 , Toshi Nagata 4 , Minoru Yoneda 3 , Negar Haghipour 1, 6 , Timothy I. Eglinton 1 , Naohiko Ohkouchi 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-09-16 00:00:00 , DOI: 10.1021/acs.analchem.8b02693 Naoto F. Ishikawa 1, 2 , Yu Itahashi 2, 3 , Thomas M. Blattmann 1 , Yoshinori Takano 2 , Nanako O. Ogawa 2 , Masako Yamane 2, 4, 5 , Yusuke Yokoyama 4 , Toshi Nagata 4 , Minoru Yoneda 3 , Negar Haghipour 1, 6 , Timothy I. Eglinton 1 , Naohiko Ohkouchi 2
Affiliation
|
We have improved a method for isolation and purification of individual amino acids for compound-specific radiocarbon analysis (CSRA). To remove high-performance liquid chromatography (HPLC) eluent blanks from isolated amino acid fractions prior to the radiocarbon (Δ14C) measurement, each fraction was filtered through a membrane filter and then washed with diethyl ether twice. Radiocarbon measurements on standard amino acids processed and purified with the above method using elemental analyzer–accelerator mass spectrometry resulted in Δ14C values that were in strong agreement (R2 = 0.998) with the original Δ14C value of each amino acid standard. From these measurements, we calculate dead and modern carbon contamination contributions as 1.2 ± 0.2 and 0.3 ± 0.1 μgC, respectively, which are consistent with direct assessments of HPLC procedural blanks of 1.0 ± 0.8 μgC per sample. These contamination constraints allow correction of measured Δ14C values for accurate and precise CSRA and are widely applicable to future archeological and biogeochemical studies.
中文翻译：
用于放射性碳分析的衍生化氨基酸的分离和纯化的改进方法
我们已经改进了分离和纯化用于化合物特异性放射性碳分析（CSRA）的单个氨基酸的方法。为了从分离前的放射性碳氨基酸级分除去高效液相色谱法（HPLC）洗脱剂坯件（Δ 14 C）的测量，每级分通过膜滤器过滤，然后用二乙醚洗涤两次。在标准的氨基放射性碳测量酸处理并与使用元素分析仪加速器质谱上述方法纯化导致Δ 14那名在强协议（C值- [R 2 = 0.998）与原来的Δ 14每个氨基酸标准的C值。通过这些测量，我们计算出的死碳和现代碳污染贡献分别为1.2±0.2和0.3±0.1μgC，这与对每个样品1.0±0.8μgC的HPLC程序空白的直接评估相一致。这些污染约束允许测量Δ的修正14用于准确和精确CSRA C值和广泛适用于未来的考古和生物地球化学研究。
更新日期：2018-09-16
中文翻译：
用于放射性碳分析的衍生化氨基酸的分离和纯化的改进方法
我们已经改进了分离和纯化用于化合物特异性放射性碳分析（CSRA）的单个氨基酸的方法。为了从分离前的放射性碳氨基酸级分除去高效液相色谱法（HPLC）洗脱剂坯件（Δ 14 C）的测量，每级分通过膜滤器过滤，然后用二乙醚洗涤两次。在标准的氨基放射性碳测量酸处理并与使用元素分析仪加速器质谱上述方法纯化导致Δ 14那名在强协议（C值- [R 2 = 0.998）与原来的Δ 14每个氨基酸标准的C值。通过这些测量，我们计算出的死碳和现代碳污染贡献分别为1.2±0.2和0.3±0.1μgC，这与对每个样品1.0±0.8μgC的HPLC程序空白的直接评估相一致。这些污染约束允许测量Δ的修正14用于准确和精确CSRA C值和广泛适用于未来的考古和生物地球化学研究。
京公网安备 11010802027423号