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Surface Modification of Cellulose Membranes To Prepare a High-Capacity Membrane Adsorber for Monoclonal Antibody Purification via Hydrophobic Charge-Induction Chromatography
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2018-09-18 , DOI: 10.1021/acs.iecr.8b02958 Na Ma 1 , Dongxue Yao 1 , Hui Yang 1 , Jian Yin 1 , Hua Wang 1 , Yufeng Zhang 2 , Jianqiang Meng 1
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2018-09-18 , DOI: 10.1021/acs.iecr.8b02958 Na Ma 1 , Dongxue Yao 1 , Hui Yang 1 , Jian Yin 1 , Hua Wang 1 , Yufeng Zhang 2 , Jianqiang Meng 1
Affiliation
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Hydrophobic charge-induction chromatography (HCIC) has emerged as an efficient method for antibody purification, but traditional packed-bed chromatography continues to suffer from high pressure drop and low transport efficiency. A versatile surface modification method is proposed to prepare HCIC membrane adsorbers. We first modified a commercial regenerated cellulose (RC) membrane by the cationic ring-opening polymerization (CROP) of diethylene glycol diglycidyl ether (DEGDE), producing the RC-g-PDEGDE membrane, which was then modified by the ring-opening reaction of an epoxy group with a sulfydryl group in four mercaptoheterocyclic ligands. Bovine serum albumin (BSA) and human immunoglobulin G (IgG) were used as model contaminant and antibody, respectively. The highest ligand density achieved is 370 mmol/L, and the static and dynamic adsorption capacities of IgG reached 195 and 65 mg/mL, respectively. The high dynamic capacity and IgG recovery (96%) are believed to benefit from the branched structure of DEGDE and convective transport in membrane pores.
中文翻译：
纤维素膜的表面修饰，以制备高容量的膜吸附器，用于通过疏水性电荷诱导色谱法纯化单克隆抗体
疏水电荷诱导色谱法（HCIC）已经成为一种有效的抗体纯化方法，但是传统的填充床色谱法仍然遭受高压降和低运输效率的困扰。提出了一种通用的表面改性方法来制备HCIC膜吸附器。我们首先通过二甘醇二缩水甘油醚（DEGDE）的阳离子开环聚合（CROP）改性了商用再生纤维素（RC）膜，从而生产出RC- g-PDEGDE膜，然后通过环氧基与四个巯基杂环配体中的巯基的开环反应进行修饰。牛血清白蛋白（BSA）和人免疫球蛋白G（IgG）分别用作模型污染物和抗体。达到的最高配体密度为370 mmol / L，IgG的静态和动态吸附容量分别达到195和65 mg / mL。据信，高动态容量和IgG回收率（96％）得益于DEGDE的分支结构和膜孔中的对流传输。
更新日期：2018-09-19
中文翻译：
纤维素膜的表面修饰，以制备高容量的膜吸附器，用于通过疏水性电荷诱导色谱法纯化单克隆抗体
疏水电荷诱导色谱法（HCIC）已经成为一种有效的抗体纯化方法，但是传统的填充床色谱法仍然遭受高压降和低运输效率的困扰。提出了一种通用的表面改性方法来制备HCIC膜吸附器。我们首先通过二甘醇二缩水甘油醚（DEGDE）的阳离子开环聚合（CROP）改性了商用再生纤维素（RC）膜，从而生产出RC- g-PDEGDE膜，然后通过环氧基与四个巯基杂环配体中的巯基的开环反应进行修饰。牛血清白蛋白（BSA）和人免疫球蛋白G（IgG）分别用作模型污染物和抗体。达到的最高配体密度为370 mmol / L，IgG的静态和动态吸附容量分别达到195和65 mg / mL。据信，高动态容量和IgG回收率（96％）得益于DEGDE的分支结构和膜孔中的对流传输。
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