Examination of the Deubiquitylation Site Selectivity of USP51 by Using Chemically Synthesized Ubiquitylated Histones,ChemBioChem

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Examination of the Deubiquitylation Site Selectivity of USP51 by Using Chemically Synthesized Ubiquitylated Histones
ChemBioChem ( IF 2.6 ) Pub Date : 2018-11-21 , DOI: 10.1002/cbic.201800432
Huasong Ai ₁ , Yu Guo _{2,

3} , Demeng Sun ₄ , Sanling Liu ₄ , Yunkun Qi ₁ , Jing Guo ₁ , Qian Qu ₁ , Qingyue Gong ₄ , Suwen Zhao ₂ , Jiabin Li ₄ , Lei Liu ₁

Affiliation

Nicking nucleosomes: Five ubiquitylated histones are synthesized through chemical total synthesis and reconstituted to nucleosomes. These histones are used to examine the deubiquitylation selectivity of USP51. Results indicate that USP51 cleaves the surface‐ubiquitylated nucleosomes, but is inactive against ubiquitin embedded inside the nucleosome.