使用光活化电化学技术在Si（100）表面进行DNA杂交检测：牛血清白蛋白和六乙二醇作为防污层的比较研究,Langmuir

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使用光活化电化学技术在Si（100）表面进行DNA杂交检测：牛血清白蛋白和六乙二醇作为防污层的比较研究
Langmuir ( IF 3.7 ) Pub Date : 2018-09-05 00:00:00 , DOI: 10.1021/acs.langmuir.8b02222
Leila Zarei ₁ , Roya Tavallaie ₁ , Moinul H. Choudhury ₁ , Stephen G. Parker ₁ , Padmavathy Bakthavathsalam ₁ , Simone Ciampi ₂ , Vinicius R. Gonçales ₁ , J. Justin Gooding ₁

Affiliation

光可用于在空间上解析半导体电极上的电化学测量结果。已经探索了这种现象，以检测可光寻址的电位传感器和最近的基于有机单层保护的Si（100）的可光寻址安培传感器的DNA杂交。在这里，通过比较使用牛血清白蛋白（BSA）和六甘醇（OEG ₆）作为防污层来抵抗对Si（100）装置上的DNA修饰界面的非特异性吸附的传感性能，来对这一领域做出贡献。观察到的是，两个基于BSA或OEG _6的传感器最初都允许互补，非互补和错配DNA靶之间的电化学区分。但是，仅基于OEG的曲面₆可以随时间维持电活性。我们的研究结果表明，这涉及到加速的SiO _X形成引起的由BSA蛋白质吸附在单分子层保护的Si（100）表面。因此，在OEG ₆作为防污层在分子水平上进行修饰的低掺杂Si（100）电极上进行了DNA生物传感器的分析研究。首先，在一系列互补的DNA靶标浓度范围内记录了光激活的电化学反应。线性半对数关系从1.0×10 ^–11到1.0×10 ^–6 mol L ^–1相关系数为0.942。然后，用三个独立的表面进行测量表明相对标准偏差为4.5％。最后，在复杂样品中成功进行了选择性测试，该样品由四种不同DNA序列的混合物混合而成。在一起，这些结果表明，通过使用OEG ₆作为防污层，可以在Si（100）上实现可靠且稳定的光激活电流型DNA传感器。

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DNA-Hybridization Detection on Si(100) Surfaces Using Light-Activated Electrochemistry: A Comparative Study between Bovine Serum Albumin and Hexaethylene Glycol as Antifouling Layers

Light can be used to spatially resolve electrochemical measurements on a semiconductor electrode. This phenomenon has been explored to detect DNA hybridization with light-addressable potentiometric sensors and, more recently, with light-addressable amperometric sensors based on organic-monolayer-protected Si(100). Here, a contribution to the field is presented by comparing sensing performances when bovine serum albumin (BSA) and hexaethylene glycol (OEG₆) are employed as antifouling layers that resist nonspecific adsorption to the DNA-modified interface on Si(100) devices. What is observed is that both sensors based on BSA or OEG₆ initially allow electrochemical distinction among complementary, noncomplementary, and mismatched DNA targets. However, only surfaces based on OEG₆ can sustain electroactivity over time. Our results suggest that this relates to accelerated SiO_x formation occasioned by BSA proteins adsorbing on monolayer-protected Si(100) surfaces. Therefore, DNA biosensors were analytically explored on low-doped Si(100) electrodes modified on the molecular level with OEG₆ as an antifouling layer. First, light-activated electrochemical responses were recorded over a range of complementary DNA target concentrations. A linear semilog relation was obtained from 1.0 × 10^–11 to 1.0 × 10^–6 mol L^–1 with a correlation coefficient of 0.942. Then, measurements with three independent surfaces indicated a relative standard deviation of 4.5%. Finally, selectivity tests were successfully performed in complex samples consisting of a cocktail mixture of four different DNA sequences. Together, these results indicate that reliable and stable light-activated amperometric DNA sensors can be achieved on Si(100) by employing OEG₆ as an antifouling layer.

更新日期：2018-09-05

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