三联吡啶钌（II）衍生物的自增强电化学发光纳米棒及其在检测<i>N-</i>乙酰基-β-d-氨基葡萄糖苷酶中的传感应用,Analytical Chemistry

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三联吡啶钌（II）衍生物的自增强电化学发光纳米棒及其在检测N-乙酰基-β-d-氨基葡萄糖苷酶中的传感应用
Analytical Chemistry ( IF 6.7 ) Pub Date : 2016-01-29 00:00:00 , DOI: 10.1021/acs.analchem.5b03954
Haijun Wang ₁ , Yali Yuan ₁ , Ying Zhuo ₁ , Yaqin Chai ₁ , Ruo Yuan ₁

Affiliation

通过共价连接双（2,2'-联吡啶基）（4'-甲基-[2,2']联吡啶基-4-羧酸）钌（II）（Ru（bpy））合成的自增强电化学发光（ECL）试剂具有三（3-氨基丙基）胺（TAPA）的₂（mcbpy）²⁺）已被选作前驱体，以制备高效率的纳米棒（[Ru（bpy）₂（mcbpy）²⁺ -TAPA] NRs）。溶剂蒸发诱导的自组装程序。由于较短的电子传输路径和较少的能量损失，发光Ru（bpy）₂（mcbpy）²⁺之间的分子内反应与常见的分子间ECL反应相比，TAPA中的具有核心活性的叔胺基团显示出更高的发光效率。此外，使用电化学发光的Ru（II）基络合物作为前驱体直接制备具有高电活性表面积的纳米结构是一种更有效，更方便的方法，用于提高Ru（II）基络合物的固定化量。生物传感器与传统的固定方法相比。同时，由于它们的正电性质和表面上的氨基，所得到的纳米棒可以容易地进一步官能化。在这里，功能化的[Ru（bpy）₂（mcbpy）²⁺ -TAPA] NRs的Pt纳米颗粒用于加载检测抗体（Ab ₂）。另外，合成具有分级分支结构的Au / Pd树状聚合物（DR）以固定的量固定捕获抗体（Ab ₁）。基于夹心免疫反应，构建了一种简单而灵敏的“信号开启”免疫传感器，用于检测糖尿病性肾病的生物标志物N-乙酰-β - d-氨基葡萄糖苷酶（NAG），浓度线性良好，线性范围从1 ng mL ^{– 1}至0.5 pg mL ^–1的检出限和0.17 pg mL ^–1的检出限。

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Self-Enhanced Electrochemiluminescence Nanorods of Tris(bipyridine) Ruthenium(II) Derivative and Its Sensing Application for Detection of N-Acetyl-β-d-glucosaminidase

A self-enhanced electrochemiluminescence (ECL) reagent, synthesized by covalently linking bis(2,2′-bipyridyl)(4′-methyl-[2,2′]bipyridinyl-4-carboxylicacid) ruthenium(II) (Ru(bpy)₂(mcbpy)²⁺) with tris(3-aminopropyl)amine (TAPA), has been chosen as precursor to prepare nanorods ([Ru(bpy)₂(mcbpy)²⁺-TAPA]NRs) with high luminous efficiency via a solvent-evaporation-induced self-assembly procedure. Due to the shorter electron-transfer path and less energy loss, the intramolecular reaction between the luminescent Ru(bpy)₂(mcbpy)²⁺ and coreactive tertiary amine group in TAPA has shown improved luminous efficiency compared with the common intermolecular ECL reactions. Moreover, using the electrochemiluminescent Ru(II)-based complex as precursor to directly prepare a nanostructure with high electro-active surface area is a more effective and convenient method for enhancing the immobilized amount of Ru(II)-based complex in the construction of biosensors compared with the traditional immobilized methods. Meanwhile, the obtained nanorods could be further functionalized easily, owing to their positive electrical property and the amino group on the surface. Here, Pt nanoparticles functionalized [Ru(bpy)₂(mcbpy)²⁺-TAPA]NRs are used to load the detection antibody (Ab₂). In addition, the Au/Pd dendrimers (DRs) with hierarchically branched structures are synthesized to immobilize capture antibody (Ab₁) with increased amount. Based on sandwiched immunoreactions, a simple and sensitive “signal-on” immunosensor is constructed for the detection of N-acetyl-β-d-glucosaminidase (NAG), a biomarker of diabetic nephropathy, with excellent linearity in concentrations from 1 ng mL^–1 to 0.5 pg mL^–1 and a detection limit of 0.17 pg mL^–1.

更新日期：2016-01-29

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