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Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2018-08-29 , DOI: 10.1021/jacs.8b07073
Tae-Il Kim 1 , Byunghee Hwang 1 , Boeun Lee 2 , Jeehyeon Bae 3 , Youngmi Kim 1
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2018-08-29 , DOI: 10.1021/jacs.8b07073
Tae-Il Kim 1 , Byunghee Hwang 1 , Boeun Lee 2 , Jeehyeon Bae 3 , Youngmi Kim 1
Affiliation
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The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.
中文翻译:
用 J 聚集探针选择性监测和成像嗜酸性粒细胞过氧化物酶活性
嗜酸性粒细胞过氧化物酶 (EPO) 活性的特异性检测需要难以区分 EPO 产生的次溴酸和其他卤代过氧化物酶产生的次氯酸。在这里,我们报告了一种对 HOBr 比 HOCl 具有高动力学选择性 (≥1200:1) 卤化的荧光探针。重原子效应不会淬灭二溴化产物,因为它会自组装成提供开启信号的发射 J 聚集体。报告了这种荧光剂在 EPO 活性测定、试纸传感器、EPO 活性荧光成像、癌细胞氧化应激测定和活小鼠免疫反应检测中的应用。
更新日期:2018-08-29
中文翻译:
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用 J 聚集探针选择性监测和成像嗜酸性粒细胞过氧化物酶活性
嗜酸性粒细胞过氧化物酶 (EPO) 活性的特异性检测需要难以区分 EPO 产生的次溴酸和其他卤代过氧化物酶产生的次氯酸。在这里,我们报告了一种对 HOBr 比 HOCl 具有高动力学选择性 (≥1200:1) 卤化的荧光探针。重原子效应不会淬灭二溴化产物,因为它会自组装成提供开启信号的发射 J 聚集体。报告了这种荧光剂在 EPO 活性测定、试纸传感器、EPO 活性荧光成像、癌细胞氧化应激测定和活小鼠免疫反应检测中的应用。