Carbon Nanotube-Polyamidoamine Dendrimer Hybrid-Modified Electrodes for Highly Sensitive Electrochemical Detection of MicroRNA24,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Carbon Nanotube-Polyamidoamine Dendrimer Hybrid-Modified Electrodes for Highly Sensitive Electrochemical Detection of MicroRNA24
Analytical Chemistry ( IF 6.7 ) Pub Date : 2015-04-24 00:00:00 , DOI: 10.1021/acs.analchem.5b00093
Fengye Li ₁ , Jing Peng ₁ , Qiong Zheng ₁ , Xiang Guo ₁ , Hao Tang ₁ , Shouzhuo Yao _{1,

2}

Affiliation

A simple and ultrasensitive microRNA (miRNA) electrochemical biosensor employing multiwalled carbon nanotube (MWCNT)-polyamidoamine (PAMAM) dendrimer and methylene blue (MB) redox indicator is reported in this work. The assay utilizes a glass carbon (GC) electrode modified with MWCNT-PAMAM, on which the oligonucleotide capture probes are immobilized. The electrochemical detection of miRNAs is completed by measuring the reduction signal change of MB before and after the probe hybridization with target miRNA (miRNA24 is used as a model case). The MWCNT-PAMAM/GC electrode shows greatly enhanced signal to MB reduction in contrast to bare GC electrode. The functionalization of MWCNT with PAMAM maintains the electrochemical property of MWCNT to MB reduction but minimizes the undesired adsorption of MB on the MWCNT surface. The effect of experimental variables on the miRNA detection is investigated and optimized. A detection limit of 0.5 fM and a linear peak current density-concentration relationship up to 100 nM are obtained following 60 min hybridization. The proposed assay is successfully used to detect miRNA24 in total RNA sample extracted from HeLa cells.

中文翻译：

碳纳米管-聚酰胺酰胺树状聚合物杂交修饰电极用于MicroRNA24的高灵敏电化学检测。

在这项工作中报道了一种简单，超灵敏的microRNA（miRNA）电化学生物传感器，该传感器采用了多壁碳纳米管（MWCNT）-聚酰胺基胺（PAMAM）树枝状聚合物和亚甲基蓝（MB）氧化还原指示剂。该测定利用修饰有MWCNT-PAMAM的玻璃碳（GC）电极，寡核苷酸捕获探针固定在该电极上。通过测量与目标miRNA探针杂交之前和之后MB的还原信号变化，可以完成miRNA的电化学检测（miRNA24被用作模型案例）。与裸GC电极相比，MWCNT-PAMAM / GC电极显示出MB减少的信号大大增强。用PAMAM对MWCNT进行官能化，可以保持MWCNT还原MB的电化学性能，但可以将MB在MWCNT表面上的不良吸附降至最低。研究和优化了实验变量对miRNA检测的影响。杂交60分钟后，检测限为0.5 fM，线性峰值电流密度-浓度关系高达100 nM。拟议的测定法已成功用于检测从HeLa细胞提取的总RNA样品中的miRNA24。

更新日期：2015-04-24

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南