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“Signal-On” Photoelectrochemical Biosensor for Sensitive Detection of Human T-Cell Lymphotropic Virus Type II DNA: Dual Signal Amplification Strategy Integrating Enzymatic Amplification with Terminal Deoxynucleotidyl Transferase-Mediated Extension
Analytical Chemistry ( IF 6.7 ) Pub Date : 2015-04-24 00:00:00 , DOI: 10.1021/acs.analchem.5b00679
Qingming Shen 1 , Li Han 1 , Gaochao Fan 2 , Jian-Rong Zhang 2 , Liping Jiang 2 , Jun-Jie Zhu 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2015-04-24 00:00:00 , DOI: 10.1021/acs.analchem.5b00679
Qingming Shen 1 , Li Han 1 , Gaochao Fan 2 , Jian-Rong Zhang 2 , Liping Jiang 2 , Jun-Jie Zhu 2
Affiliation
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A novel “signal-on” photoelectrochemical (PEC) biosensor for sensitive detection of human T-cell lymphotropic virus type II (HTLV-II) DNA was developed on the basis of enzymatic amplification coupled with terminal deoxynucleotidyl transferase (TdT)-mediated extension strategy. The intensity of the photocurrent signal was proportional to the concentration of the HTLV-II DNA-target DNA (tDNA) by dual signal amplification. In this protocol, GR-CdS:Mn/ZnS nanocomposites were used as photoelectric conversion material, while pDNA was used as the tDNA recognizing unit. Moreover, the TdT-mediated extension and the enzymatic signal amplification technique were used to enhance the sensitivity of detection. Using this novel dual signal amplification strategy, the prototype of PEC DNA sensor can detect as low as ∼0.033 fM of HTLV-II DNA with a linear range of 0.1–5000 fM, with excellent differentiation ability even for single-base mismatches. This PEC DNA assay opens a promising platform to detect various DNA targets at ultralow levels for early diagnoses of different diseases.
中文翻译：
“ Signal-On”光电化学生物传感器，用于敏感地检测人类T细胞淋巴病毒II型DNA：双信号放大策略，将酶促扩增与末端脱氧核苷酸转移酶介导的延伸相结合
在酶放大与末端脱氧核苷酸转移酶（TdT）介导的扩展策略的基础上，开发了一种新型的“信号开启”光电化学（PEC）生物传感器，用于敏感地检测II型人类T细胞（HTLV-II）DNA。 。通过双重信号放大，光电流信号的强度与HTLV-II DNA靶DNA（tDNA）的浓度成正比。在该协议中，GR-CdS：Mn / ZnS纳米复合材料被用作光电转换材料，而pDNA被用作tDNA识别单元。此外，TdT介导的扩展和酶信号放大技术被用来提高检测的灵敏度。使用这种新颖的双信号放大策略，PEC DNA传感器的原型可以检测低至约0。033 fM的HTLV-II DNA线性范围为0.1–5000 fM，即使对于单碱基错配也具有出色的分化能力。这种PEC DNA测定法提供了一个有前途的平台，可以以超低水平检测各种DNA靶标，以早期诊断各种疾病。
更新日期：2015-04-24
中文翻译：
“ Signal-On”光电化学生物传感器，用于敏感地检测人类T细胞淋巴病毒II型DNA：双信号放大策略，将酶促扩增与末端脱氧核苷酸转移酶介导的延伸相结合
在酶放大与末端脱氧核苷酸转移酶（TdT）介导的扩展策略的基础上，开发了一种新型的“信号开启”光电化学（PEC）生物传感器，用于敏感地检测II型人类T细胞（HTLV-II）DNA。 。通过双重信号放大，光电流信号的强度与HTLV-II DNA靶DNA（tDNA）的浓度成正比。在该协议中，GR-CdS：Mn / ZnS纳米复合材料被用作光电转换材料，而pDNA被用作tDNA识别单元。此外，TdT介导的扩展和酶信号放大技术被用来提高检测的灵敏度。使用这种新颖的双信号放大策略，PEC DNA传感器的原型可以检测低至约0。033 fM的HTLV-II DNA线性范围为0.1–5000 fM，即使对于单碱基错配也具有出色的分化能力。这种PEC DNA测定法提供了一个有前途的平台，可以以超低水平检测各种DNA靶标，以早期诊断各种疾病。
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