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Soybean Glycinin- and β-Conglycinin-Induced Intestinal Damage in Piglets via the p38/JNK/NF-κB Signaling Pathway
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-08-24 00:00:00 , DOI: 10.1021/acs.jafc.8b03641 Chenglu Peng 1 , Chengming Cao 1 , Mengchu He 1 , Yingshuang Shu 1 , Xuebing Tang 1 , Yuanhong Wang 1 , Yu Zhang 1 , Xiaodong Xia 1 , Yu Li 1 , Jinjie Wu 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-08-24 00:00:00 , DOI: 10.1021/acs.jafc.8b03641 Chenglu Peng 1 , Chengming Cao 1 , Mengchu He 1 , Yingshuang Shu 1 , Xuebing Tang 1 , Yuanhong Wang 1 , Yu Zhang 1 , Xiaodong Xia 1 , Yu Li 1 , Jinjie Wu 1
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β-Conglycinin (7S) and glycinin (11S) are known to induce a variety of hypersensitivity reactions involving the skin, intestinal tract, and respiratory tract. The present study aimed to identify the mechanism underlying the development of allergy to soybean antigen proteins, using piglets as an animal model. Weaned “Duroc × Landrace × Yorkshire” piglets were fed a diet supplemented with 7S or 11S to investigate the signaling pathway involved in intestinal damage in piglets. Results showed that serum nitric oxide (NO), tumor necrosis factor-α (TNF-α), and caspase-3 levels were significantly higher in 7S- and 11S-fed piglets compared to those in suckling or weaned ones. mRNA, protein, and phosphorylation levels of nuclear factor-kappa B (NF-κB), p38, and Jun N-terminal kinase (JNK) were higher in 7S- and 11S-fed piglets than in suckling and weaned ones. Overall, our results indicate that 7S and 11S damaged the intestinal function in piglets through their impact on NF-κB, JNK, and p38 expression.
中文翻译：
大豆大豆球蛋白和 β-伴大豆球蛋白通过 p38/JNK/NF-κB 信号通路诱导仔猪肠道损伤
β-伴大豆球蛋白 (7S) 和大豆球蛋白 (11S) 已知可诱发多种涉及皮肤、肠道和呼吸道的过敏反应。本研究旨在以仔猪为动物模型，以确定对大豆抗原蛋白过敏的发生机制。断奶的“杜洛克×长白×约克夏”仔猪饲喂添加7S或11S的日粮，以研究仔猪肠道损伤中涉及的信号通路。结果显示，7S 和 11S 喂养仔猪的血清一氧化氮 (NO)、肿瘤坏死因子-α (TNF-α) 和 caspase-3 水平显着高于哺乳期或断奶仔猪。 7S 和 11S 饲喂仔猪的核因子 kappa B (NF-κB)、p38 和 Jun N 末端激酶 (JNK) 的 mRNA、蛋白质和磷酸化水平高于哺乳仔猪和断奶仔猪。总体而言，我们的结果表明 7S 和 11S 通过影响 NF-κB、JNK 和 p38 表达而损害仔猪的肠道功能。
更新日期：2018-08-24
中文翻译：
大豆大豆球蛋白和 β-伴大豆球蛋白通过 p38/JNK/NF-κB 信号通路诱导仔猪肠道损伤
β-伴大豆球蛋白 (7S) 和大豆球蛋白 (11S) 已知可诱发多种涉及皮肤、肠道和呼吸道的过敏反应。本研究旨在以仔猪为动物模型，以确定对大豆抗原蛋白过敏的发生机制。断奶的“杜洛克×长白×约克夏”仔猪饲喂添加7S或11S的日粮，以研究仔猪肠道损伤中涉及的信号通路。结果显示，7S 和 11S 喂养仔猪的血清一氧化氮 (NO)、肿瘤坏死因子-α (TNF-α) 和 caspase-3 水平显着高于哺乳期或断奶仔猪。 7S 和 11S 饲喂仔猪的核因子 kappa B (NF-κB)、p38 和 Jun N 末端激酶 (JNK) 的 mRNA、蛋白质和磷酸化水平高于哺乳仔猪和断奶仔猪。总体而言，我们的结果表明 7S 和 11S 通过影响 NF-κB、JNK 和 p38 表达而损害仔猪的肠道功能。
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