Cultures of melanized fungi representative of the black yeast orders Capnodiales (Cladosporium cladosporioides and Neohortaea acidophila) and Chaetothyriales (Cladophialophora psammophila) were confined with indoor air from the laboratory during 48 hours. Volatile organic compounds (VOCs) from the headspace were analyzed by thermal desorption gas chromatography time-of-fly mass spectrometry (TD-GC-ToFMS, detection threshold 0.1 µg·m^-3) and compared against an abiotic control. A mixture of 71 VOCs were identified and quantified in the indoor air (total concentration 1.4 mg m^-3). Most of these compounds were removed in the presence of fungal biomass, but 42 newly formed putative volatile metabolites were detected though at comparatively low total concentrations (<50 µg·m^-3). The VOCs emission profile of C. cladosporioides, a ubiquitous and well-known species often associated to the sick building syndrome, was consistent with previous literature reports. The specialized C. psammophila and N. acidophila, isolated respectively from gasoline polluted soil and from lignite, displayed rather specific VOCs emission profiles. Mass balances on the fungal uptake and generation of VOCs resulted in overall VOCs removal efficiencies higher than 96% with all tested fungi. Applied aspects and biosafety issues concerning the suitability of black yeasts for the biofiltration of indoor air have been discussed.

在48小时内，来自实验室的室内空气限制了代表黑色酵母菌Capnodiales（Cladosporium cladosporioides和Neohortaea acidophila）和Chaetothyriales（Cladophialophora psammophila）的黑色化真菌的培养。用热脱附气相色谱飞行时间质谱（TD-GC-ToFMS，检测阈值为0.1 µg·m ^-3）分析顶空中的挥发性有机化合物（VOC），并与非生物对照进行比较。在室内空气中鉴定并定量了71种VOC的混合物（总浓度1.4 mg m ^-3）。在真菌生物质存在的情况下，大多数这些化合物都被去除了，尽管总浓度较低（<50 µg·m ^-3），但仍检测到42种新形成的挥发性代谢物。C. cladosporioides（一种通常与病态建筑综合症有关的普遍存在的物种）的VOCs排放特征与以前的文献报道一致。专业的嗜肺梭状芽胞杆菌和嗜酸N.分别从汽油污染的土壤和褐煤中分离出来的，表现出相当特殊的VOC排放特征。在所有测试真菌中，真菌吸收和产生VOC的质量平衡导致整体VOC的去除效率高于96％。已经讨论了涉及黑酵母对室内空气进行生物过滤的适用性方面和生物安全问题。