Dual Electrolytic Eluent Generation for Oligosaccharides Analysis Using High-Performance Anion-Exchange Chromatography,Analytical Chemistry

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Dual Electrolytic Eluent Generation for Oligosaccharides Analysis Using High-Performance Anion-Exchange Chromatography
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-08-14 00:00:00 , DOI: 10.1021/acs.analchem.8b02436
Yongjing Chen ₁ , Victor Barreto ₁ , Andy Woodruff ₁ , Zhongqing Lu ₁ , Yan Liu ₁ , Christopher Pohl ₁

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The research on oligosaccharides is growing and gaining in importance at a rapid pace. The efforts to understand their bioactivity and to develop new products based on oligosaccharides in biotherapeutics and food industry require effective and reliable tools for analysis of oligosaccharides. Here we present a dual electrolytic eluent generation platform for the analysis of oligosaccharides by high-performance anion-exchange liquid chromatography (HPAE) in both analytical and capillary column formats. The system consists of one eluent generator producing methanesulfonic acid (MSA) connected in series with a second eluent generator producing potassium hydroxide (KOH). Through manipulating the concentration output of both eluent generators, chromatographic performance comparable to that obtained using the conventional sodium acetate/sodium hydroxide (NaOAc/NaOH) eluents is achieved using the electrolytically generated potassium methanesulfonate/potassium hydroxide (KMSA/KOH) eluent. This platform utilizes deionized water as the only carrier stream through a single isocratic pump, overcomes the various drawbacks associated with manually prepared NaOAc/NaOH eluents, and offers an easy to use, simplified operation solution for oligosaccharides profiling with increased precision and accuracy.

中文翻译：

高效阴离子交换色谱法用于寡糖分析的双电解洗脱液生成

寡糖的研究正在迅速发展并变得越来越重要。在生物治疗学和食品行业中，了解其生物活性并开发基于寡糖的新产品的努力需要有效且可靠的工具来进行寡糖分析。在这里，我们介绍了一种双电解洗脱液生成平台，用于通过高效阴离子交换液相色谱（HPAE）以分析和毛细管柱形式分析寡糖。该系统由一个产生甲烷磺酸（MSA）的洗脱液发生器与另一个产生氢氧化钾（KOH）的洗脱液发生器串联连接。通过操纵两个洗脱液发生器的浓度输出，使用电解生成的甲磺酸钾/氢氧化钾（KMSA / KOH）洗脱液可实现与常规乙酸钠/氢氧化钠（NaOAc / NaOH）洗脱液相当的色谱性能。该平台利用去离子水作为通过单个等度泵的唯一载流，克服了与手动制备的NaOAc / NaOH洗脱液相关的各种弊端，并提供了易于使用的简化操作解决方案，用于寡糖分析，具有更高的精度和准确性。

更新日期：2018-08-14

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