DNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG° of DNA motifs at temperatures and buffer conditions of interest, with significantly better accuracy (6- to 14-fold lower s.e.) than prior methods. The equilibrium constant of a reaction with thermodynamics closely approximating that of a desired motif is numerically calculated from directly observed reactant and product equilibrium concentrations; a DNA catalyst is designed to accelerate equilibration. We measured the ΔG° of terminal fluorophores, single-nucleotide dangles and multinucleotide dangles, in temperatures ranging from 10 to 45 °C.

中文翻译：

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通过非共价催化自然表征核酸基序热力学。

DNA杂交热力学对于生物技术和纳米技术应用的寡核苷酸的精确设计至关重要，但是基于对热行为的有限定量理解，目前正在使用的参数是不准确地推断出来的。在这里，我们提出了一种在感兴趣的温度和缓冲液条件下测量DNA基序的ΔG°的方法，其准确性（比以往低6至14倍）。根据直接观察到的反应物和产物的平衡浓度，通过数值计算出具有接近于所需基序的热力学的反应平衡常数。DNA催化剂旨在加速平衡。我们在10至45°C的温度范围内测量了末端荧光团，单核苷酸悬挂物和多核苷酸悬挂物的ΔG°。