小麦胚芽凝集素修饰的磁性氧化铁纳米复合物，作为杀死乳腺癌细胞的细胞膜特异性受体靶材料<a href="#fn1" title="可用电子补充信息（ESI）：合成NC1，NC2和NC3的程序。 NC3的磁化曲线和PXRD图谱，纯化的WGA的表征，NC4和NC5的荧光显微镜图像，NC5在不同pH介质和10％FBS中的稳定性，比较NC5在不同细胞中的细胞活力以及细胞照片培养皿保持在磁体上，并通过荧光显微镜观察细胞对NC3的内在化。参见DOI：10.1039 / c8tb01170b">†</a>,Journal of Materials Chemistry B

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小麦胚芽凝集素修饰的磁性氧化铁纳米复合物，作为杀死乳腺癌细胞的细胞膜特异性受体靶材料 †
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-08-07 00:00:00 , DOI: 10.1039/c8tb01170b
Aekta Upadhyay _{1,

2,

3,

4,

5} , Ravinder Kandi _{1,

2,

3,

4,

5} , Chebrolu Pulla Rao _{1,

2,

3,

4,

5}

Affiliation

凝集素因其对碳水化合物结合的特异性而闻名。但是，一些特定的碳水化合物残基在癌细胞中过度表达，这对于使用特异于此类残基的凝集素可能是一个优势。在这里，我们报告了小麦胚芽凝集素（WGA）和荧光灯，FITC固定在Fe ₃ O ₄ NPs上作为乳腺癌细胞的细胞膜特异性受体靶的战略设计。，MCF-7和MDA-MB-231。通过使用显微镜和光谱学对得到的纳米复合物进行了很好的表征。WGA标记的纳米复合物进一步加载了抗癌药物5-氟尿嘧啶（5-FU），以选择性杀死癌细胞。5-FU的负载效率约为356μgmg ^-1。纳米复合物本身对所研究的所有四种细胞系（HEK，HeLa，MCF-7和MDA-MB-231）显示出约90％的细胞活力，因此是合适的靶向药物递送载体。但是，与非特异性细胞（HeLa和HEK）。纳米复合物的内在化由荧光显微镜和共焦激光扫描显微镜技术通过跟踪荧光炬FITC来支持。与非特异性细胞相比，纳米复合物在特定细胞中的内在化能力高约2倍。观察到，与不存在磁场的情况相比，当MDA-MB-231细胞暴露于磁场24小时时，内在化程度提高了约2倍。负载5-FU的纳米复合物的活死细胞测定是通过碘化丙啶（PI）染色进行的。当细胞在磁场下（从PI摄取中测量）时，细胞死亡增加了约2.5倍。纳米复合物的这种策略性设计可以导致开发更好的方法，该方法通过充当载体来递送合适的药物来选择性地靶向和杀死癌细胞，其结果是医学领域可以受益。

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Wheat germ agglutinin modified magnetic iron oxide nanocomplex as a cell membrane specific receptor target material for killing breast cancer cells†

Lectins are known for their specificity for carbohydrate binding. However, a few specific carbohydrate residues are over expressed in cancer cells, which may be an advantage for using a lectin that is specific to such residues. Herein, we report the strategic design of wheat germ agglutinin (WGA) and a fluorescent torch, FITC immobilized on Fe₃O₄ NPs as a cell membrane specific receptor target for breast cancer cells, viz., MCF-7 and MDA-MB-231. The resultant nanocomplexes were well characterized by using microscopy and spectroscopy. The WGA tagged nanocomplex was further loaded with the anticancer drug 5-fluorouracil (5-FU) to selectively kill the cancer cells. The loading efficiency of 5-FU is ∼356 μg mg⁻¹. The nanocomplex itself shows ∼90% cell viability for all the four cell lines (HEK, HeLa, MCF-7 and MDA-MB-231) studied and is therefore a suitable targeting drug delivery vehicle. However, the nanocomplex loaded with {WGA + 5-FU} shows a ∼1.5-fold decrease in cell viability in the case of specific cells (MCF-7 and MDA-MB-231) when compared to non-specific cells (HeLa and HEK). The internalization of the nanocomplex is supported by fluorescence microscopy and confocal laser scanning microscopy techniques by tracking with the fluorescent torch, FITC. The nanocomplex can be internalized ∼2 times more in the specific cells as compared to the non-specific cells. It is observed that the internalization is ∼2 fold increased when the MDA-MB-231 cells are exposed to a magnetic field for 24 h as compared to in the absence of a magnetic field. Live–dead cell assay of the 5-FU loaded nanocomplex was done by propidium iodide (PI) staining. There is an increase in the cell death by ∼2.5 fold when the cells are under a magnetic field as measured from the PI uptake. Such strategic designing of the nanocomplex can lead to the development of a better method to selectively target and kill the cancer cells by acting as a carrier to deliver a suitable drug, as a result of which the medical field can benefit.

更新日期：2018-08-07

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