Hydrazine and bisulfite are poisonous to the environment and human health, hence it is necessary to monitor hydrazine and bisulfite in the environment and living cells. In this study, we reported a smart fluorescent probe (CMCA) for discriminative detection of hydrazine and bisulfite. The single fluorescent probe can respond to hydrazine and bisulfite with two different sets of colorimetric and ratiometric fluorescence signals together with high selectivity, fast response and low detection limit. The discriminative sensing behaviors of the probe for hydrazine and bisulfite were ascribed to different reaction mechanisms, which were confirmed by ¹H NMR and HR-MS spectra. The probe has been successfully used to discriminatively determine hydrazine and bisulfite in industrial wastewater with good recoveries. Furthermore, the probe was employed to visualize hydrazine and bisulfite in live cells from different fluorescence channels with good performance. Therefore, this probe has great potential applications for discriminative detection of hydrazine and bisulfite in the environment and living cells.

肼和亚硫酸氢盐对环境和人体健康有毒，因此有必要监测环境和活细胞中的肼和亚硫酸氢盐。在这项研究中，我们报道了一种用于区分肼和亚硫酸氢盐的智能荧光探针（CMCA）。单个荧光探针可以用两组不同的比色和比例荧光信号响应肼和亚硫酸氢盐，同时具有高选择性，快速响应和低检测限的特点。肼和亚硫酸氢盐探针的判别感应行为归因于不同的反应机理，由¹证实。1 H NMR和HR-MS光谱。该探针已成功用于判别测定工业废水中的肼和亚硫酸氢盐，回收率高。此外，该探针还用于以良好的性能可视化来自不同荧光通道的活细胞中的肼和亚硫酸氢盐。因此，该探针在判别检测环境和活细胞中的肼和亚硫酸氢盐方面具有巨大的潜在应用。