The identification of the volatile components of the resin from Tetraclinis articulata (Vahl.) Masters and its non-polar fractions 1–5 obtained by chromatographic simplification was investigated by GC-FID and GC–MS and allowed the identification of 97.3% (resin), 95.1% (1), 97.6% (2), 99.6% (3), 88.1% (4) and 95.0% (5) of the total volatile components. The resin and its fractions were characterized by the presence of a high amount of diterpenes (87.4, 80.1, 91.0, 91.6, 65.7 and 69.1%, respectively). The major identified diterpenes were trans-ferruginol (resin: 31.6%, 1: 56.5% and 2: 48.0%), manool (3: 57.0% and 4: 42.0%) and trans-totarol (5: 36.2%). In addition, trans-communic acid was isolated together with its cis diastereoisomer from the fraction 6 of the resin and identified by spectroscopic means (1D and 2D NMR) and by comparison with literature data. The resin, its fractions 1–5, trans-communic acid and cis-trans mixture were screened for their antioxidant activity using DPPH, ABTS, reducing power, catalase and paraoxonase methods, as well as for their antibacterial, anticholinesterase, anti-tyrosinase and cytotoxic potentials. The fraction 2 displayed the highest antioxidant activity (IC₅₀ = 25 ± 1 μg/mL and 64.1 ± 0.5 μM/min/L) in DPPH and paraoxonase assays, respectively. Trans-communic acid was found to be antibacterial against Staphylococus aureus and Enterococcus faecalis (IZ = 23.9 ± 1.5 and 20.5 ± 0.5 mm, respectively). Furthermore, the resin showed the best anti-tyrosinase activity with 87.2% of inhibition at 50 μg/mL and cis-trans mixture exerted a significant cytotoxic effect against HeLa cell line (IC₅₀ = 09.5 ± 1.0 μg/mL).

通过GC-FID和GC-MS研究了从四叶草（Vahl。）母版及其通过色谱简化获得的非极性级分1-5的树脂中挥发性成分的鉴定，鉴定出97.3％（树脂） ，占总挥发性成分的95.1％（1），97.6％（2），99.6％（3），88.1％（4）和95.0％（5）。该树脂及其馏分的特征在于存在大量的二萜（分别为87.4、80.1、91.0、91.6、65.7和69.1％）。鉴定出的主要二萜是反式-铁苦丁酚（树脂：31.6％，1：56.5％和2：48.0％），马努尔（3：57.0％和4：42.0％）和反式-甲苯酚（5：36.2％）。此外，反式-communic酸用其分离一起顺树脂级分6的非对映异构体，并通过光谱学方法（1D和2D NMR）进行鉴定，并与文献数据进行比较。树脂，其级分1-5，反式-communic酸和顺式-反式混合物用DPPH，ABTS，降低功率，过氧化氢酶对氧磷酶和方法筛选其抗氧化活性，以及对它们的抗菌，抗胆碱酯酶，抗酪氨酸酶和细胞毒性潜力。馏分2 在DPPH和对氧磷酶分析中分别显示出最高的抗氧化活性（IC ₅₀ = 25±1μg/ mL和64.1±0.5μM/ min / L）。发现反式-通酸对金黄色葡萄球菌和粪肠球菌（IZ分别为23.9±1.5和20.5±0.5 mm）。此外，该树脂显示出最佳的抗酪氨酸酶活性，在50μg/ mL时抑制率为87.2％，顺式-反式混合物对HeLa细胞系具有明显的细胞毒性作用（IC ₅₀  = 09.5±1.0μg/ mL）。