当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Upconversion Fluorescent Aptasensor for Polychlorinated Biphenyls Detection Based on Nicking Endonuclease and Hybridization Chain Reaction Dual-Amplification Strategy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-23 00:00:00 , DOI: 10.1021/acs.analchem.8b02159 Yu Wang 1 , Jialei Bai 1 , Bingyang Huo 1, 2 , Shuai Yuan 1 , Man Zhang 1 , Xuan Sun 1 , Yuan Peng 1 , Shuang Li 1 , Jiang Wang 1 , Baoan Ning 1 , Zhixian Gao 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-23 00:00:00 , DOI: 10.1021/acs.analchem.8b02159 Yu Wang 1 , Jialei Bai 1 , Bingyang Huo 1, 2 , Shuai Yuan 1 , Man Zhang 1 , Xuan Sun 1 , Yuan Peng 1 , Shuang Li 1 , Jiang Wang 1 , Baoan Ning 1 , Zhixian Gao 1
Affiliation
|
A novel upconversion fluorescent aptasensor based on hybridization chain reaction and nicking endonuclease has been developed for detection of polychlorinated biphenyls (PCBs). It combined the dual advantages of UCNPs and HCR. Two harpins (H1 and H2) were first designed according to the partial complementary sequence (cDNA) of the PCB72/106. Since the aptamer specifically recognized the target, the cDNA was detached from the magnetic microspheres (MMPs). The cDNA could initiate hybridization chain reaction (HCR) and open the stems of H1 and H2. After the addition of nicking endonuclease, UCNPs were further away from the quenchers (BHQ-1). Hence, the fluorescence intensity of upconversion nanoparticals (UCNPs) could be restored via fluorescence resonance energy transfer (FRET). Therefore, the fluorescence of UCNPs was directly proportional to concentration of PCB72/106, which was the basis for the quantification of PCB72/106. PCB72/106 could be analyzed within the ranges of 0.004 to 800 ng/mL with a detection limit of 0.0035 ng/mL (S/N = 3). The aptasensor was also used for the detection of water and soil samples, and the average recoveries ranged from 93.4% to 109.7% and 83.2% to 118.5%, respectively. The relative standard deviations (RSDs) were all below 3.2%. The signal was first amplified through HCR and further amplified with the help of nicking endonuclease. This work also provided the opportunity to develop fluorescent aptasensors for other targets using this dual-amplification strategy.
中文翻译：
基于切口核酸内切酶和杂交链反应双扩增策略的上转换荧光适配体检测多氯联苯
已经开发了一种基于杂交链反应和切口内切核酸酶的新型上转换荧光适体传感器，用于检测多氯联苯（PCBs）。它结合了UCNP和HCR的双重优势。首先根据PCB72 / 106的部分互补序列（cDNA）设计两个harpin（H1和H2）。由于适体特异性识别靶标，因此将cDNA从磁性微球（MMP）分离。cDNA可以启动杂交链反应（HCR）并打开H1和H2的茎。添加切口内切核酸酶后，UCNPs进一步远离淬灭剂（BHQ-1）。因此，可以通过荧光共振能量转移（FRET）恢复上转换纳米颗粒（UCNPs）的荧光强度。所以，UCNPs的荧光与PCB72 / 106的浓度成正比，这是定量PCB72 / 106的基础。可以在0.004至800 ng / mL的范围内分析PCB72 / 106，检出限为0.0035 ng / mL（S / N = 3）。aptasensor还用于检测水和土壤样品，平均回收率分别为93.4％至109.7％和83.2％至118.5％。相对标准偏差（RSD）均低于3.2％。信号首先通过HCR扩增，然后在切口内切核酸酶的帮助下进一步扩增。这项工作还提供了使用这种双重扩增策略开发用于其他靶标的荧光适体传感器的机会。
更新日期：2018-07-23
中文翻译：
基于切口核酸内切酶和杂交链反应双扩增策略的上转换荧光适配体检测多氯联苯
已经开发了一种基于杂交链反应和切口内切核酸酶的新型上转换荧光适体传感器，用于检测多氯联苯（PCBs）。它结合了UCNP和HCR的双重优势。首先根据PCB72 / 106的部分互补序列（cDNA）设计两个harpin（H1和H2）。由于适体特异性识别靶标，因此将cDNA从磁性微球（MMP）分离。cDNA可以启动杂交链反应（HCR）并打开H1和H2的茎。添加切口内切核酸酶后，UCNPs进一步远离淬灭剂（BHQ-1）。因此，可以通过荧光共振能量转移（FRET）恢复上转换纳米颗粒（UCNPs）的荧光强度。所以，UCNPs的荧光与PCB72 / 106的浓度成正比，这是定量PCB72 / 106的基础。可以在0.004至800 ng / mL的范围内分析PCB72 / 106，检出限为0.0035 ng / mL（S / N = 3）。aptasensor还用于检测水和土壤样品，平均回收率分别为93.4％至109.7％和83.2％至118.5％。相对标准偏差（RSD）均低于3.2％。信号首先通过HCR扩增，然后在切口内切核酸酶的帮助下进一步扩增。这项工作还提供了使用这种双重扩增策略开发用于其他靶标的荧光适体传感器的机会。
京公网安备 11010802027423号