Engineering a 3D DNA-Logic Gate Nanomachine for Bispecific Recognition and Computing on Target Cell Surfaces,Journal of the American Chemical Society

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Engineering a 3D DNA-Logic Gate Nanomachine for Bispecific Recognition and Computing on Target Cell Surfaces
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2018-07-18 , DOI: 10.1021/jacs.8b04319
Ruizi Peng ₁ , Xiaofang Zheng ₁ , Yifan Lyu _{1,

2,

3} , Liujun Xu ₁ , Xiaobing Zhang ₁ , Guoliang Ke ₁ , Qiaoling Liu ₁ , Changjun You ₁ , Shuangyan Huan ₁ , Weihong Tan _{1,

2,

3}

Affiliation

Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all recognition and computing modules within a single structure, a three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed cancer cell biomarkers with bispecific recognition. Thus, when the Boolean operator "AND" returns a true value, it is followed by an "ON" signal when the specific cell type is presented. Compared with freely dispersed double-stranded DNA (dsDNA)-based molecular circuits, this 3D DNA nanostructure, termed DNA-logic gate triangular prism (TP), showed better identification performance, enabling, in turn, better molecular targeting and fabrication of recognition nanorobotics.

中文翻译：

设计用于目标细胞表面双特异性识别和计算的 3D DNA 逻辑门纳米机器

在众多识别分子中，从细胞-SELEX 产生的 DNA 适配体表现出独特的特性，可用于识别细胞膜生物标志物，尤其是癌细胞上的蛋白质受体。为了将所有识别和计算模块集成到一个结构中，构建了一个基于三维 (3D) DNA 的逻辑门纳米机器，以通过双特异性识别来靶向过表达的癌细胞生物标志物。因此，当布尔运算符“AND”返回真值时，当出现特定单元格类型时，紧随其后的是“ON”信号。与基于自由分散的双链 DNA (dsDNA) 的分子电路相比，这种被称为 DNA 逻辑门三角棱镜 (TP) 的 3D DNA 纳米结构显示出更好的识别性能，反过来，

更新日期：2018-07-18

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