Corona phase molecular recognition (CoPhMoRe) uses a heteropolymer adsorbed onto and templated by a nanoparticle surface to recognize a specific target analyte. This method has not yet been extended to macromolecular analytes, including proteins. Herein we develop a variant of a CoPhMoRe screening procedure of single-walled carbon nanotubes (SWCNT) and use it against a panel of human blood proteins, revealing a specific corona phase that recognizes fibrinogen with high selectivity. In response to fibrinogen binding, SWCNT fluorescence decreases by >80% at saturation. Sequential binding of the three fibrinogen nodules is suggested by selective fluorescence quenching by isolated sub-domains and validated by the quenching kinetics. The fibrinogen recognition also occurs in serum environment, at the clinically relevant fibrinogen concentrations in the human blood. These results open new avenues for synthetic, non-biological antibody analogues that recognize biological macromolecules, and hold great promise for medical and clinical applications.

中文翻译：

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蛋白质靶向电晕相分子识别。

电晕相分子识别（CoPhMoRe）使用吸附在纳米颗粒表面并由纳米颗粒表面模板化的杂聚物来识别特定的目标分析物。该方法尚未扩展到包括蛋白质在内的大分子分析物。本文中，我们开发了单壁碳纳米管（SWCNT）的CoPhMoRe筛选程序的一种变体，并将其用于一系列人类血液蛋白，揭示了一种特异性电晕相，可高选择性地识别纤维蛋白原。响应纤维蛋白原结合，SWCNT荧光在饱和时降低> 80％。通过分离的亚结构域的选择性荧光猝灭提示了三个纤维蛋白原结节的顺序结合，并通过猝灭动力学进行了验证。纤维蛋白原识别也发生在血清环境中，人体血液中临床相关的纤维蛋白原浓度。这些结果为识别生物大分子的合成，非生物抗体类似物开辟了新途径，并为医学和临床应用带来了广阔前景。