Our study aimed to evaluate the toxicity of 1-hexadecyl-3-methylimidazolium chloride ([C₁₆min]Cl) on the human cervical carcinoma (Hela) cells. We evaluated toxicity, cell viability, genotoxicity, oxidative stress, apoptosis, and apoptosis-related gene expression in Hela cells following exposure to [C₁₆min]Cl. The results indicated that [C₁₆min]Cl inhibited the growth of Hela cells, decreased cell viability, induced DNA damage and apoptosis, inhibited superoxide dismutase, decreased glutathione content, as well as increased the cellular malondialdehyde level of Hela cells. Moreover, [C₁₆min]Cl induced changes in the transcription of p53, Bax and Bcl-2, suggesting that the p53 and Bcl-2 family might have been involved in the cytotoxicity and apoptosis induced by [C₁₆min]Cl in Hela cells. Taken together, these results revealed that [C₁₆min]Cl imparts oxidative stress, genotoxicity, and induces apoptosis in Hela cells; hence, it is not a green solvent.

我们的研究旨在评估1-十六烷基-3-甲基咪唑鎓氯化物（[C ₁₆ min] Cl）对人宫颈癌（Hela）细胞的毒性。我们评估了暴露于[C ₁₆ min] Cl后Hela细胞的毒性，细胞活力，遗传毒性，氧化应激，凋亡和凋亡相关基因的表达。结果表明[C ₁₆ min] Cl抑制Hela细胞的生长，降低细胞活力，诱导DNA损伤和凋亡，抑制超氧化物歧化酶，降低谷胱甘肽含量，并增加Hela细胞的细胞丙二醛水平。此外，[C ₁₆ min] Cl诱导了p53，Bax和Bcl-2转录的变化，提示p53和Bcl-2家族可能参与了[C ₁₆ min] Cl在Hela细胞中诱导的细胞毒性和凋亡。综上所述，这些结果表明[C ₁₆ min] Cl赋予Hela细胞氧化应激，遗传毒性并诱导细胞凋亡。因此，它不是绿色溶剂。