DNA水凝胶中原位合成的高度稳定和多发射性的银纳米团簇及其在羟基自由基传感中的应用,ACS Applied Materials & Interfaces

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DNA水凝胶中原位合成的高度稳定和多发射性的银纳米团簇及其在羟基自由基传感中的应用
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-07-12 00:00:00 , DOI: 10.1021/acsami.8b09152
Jing Li ₁ , Jiantao Yu ₁ , Yishun Huang ₁ , Haoran Zhao ₁ , Leilei Tian ₁

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寡核苷酸稳定的银纳米簇（AgNCs）在生物成像和生物/化学传感中显示出有希望的应用。但是，它们的光稳定性不能令人满意地限制了它们的实际应用。在这项工作中，荧光AgNCs在DNA水凝胶中原位合成，该DNA水凝胶由在存在Ag ⁺的情况下具有丰富胞嘧啶序列的交联酶促扩增的聚合DNA组成。通过合理设计DNA序列以覆盖具有可比的绿色和红色发射的广谱，优化了所得AgNC的荧光性质。在DNA水凝胶的保护下，AgNCs在周围氧气环境中显示出显着改善的光稳定性，即使在高浓度下也具有较低的细胞毒性。因此，这些性质表明，稳定的滚环扩增AgNCs是检测活细胞中活性氧/氮物质（ROS / RNS）的有希望的可能的荧光探针，因为发红光的物质易于氧化并因此转化。为绿色发光物种。最后，已证明制备的AgNCs是用于细胞成像和ROS / RNS水平监测的灵敏而特异的探针，

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Highly Stable and Multiemissive Silver Nanoclusters Synthesized in Situ in a DNA Hydrogel and Their Application for Hydroxyl Radical Sensing

Oligonucleotide-stabilized silver nanoclusters (AgNCs) show promising applications in bioimaging and bio-/chemo-sensing. However, their unsatisfactory photostability limits their practical applications. In this work, fluorescent AgNCs were synthesized in situ in a DNA hydrogel, consisting of cross-linked enzymatically amplified polymeric DNAs with cytosine-rich sequences in the presence of Ag⁺. The fluorescence property of the resultant AgNCs was optimized by a rational design of the DNA sequences to cover a broad spectrum with comparable green and red emissions. Under the protection of the DNA hydrogel, the AgNCs showed significantly improved photostability in an ambient oxygen environment, as well as low cytotoxicity even at a high concentration. Therefore, these properties show the rolling-circle-amplification-stabilized AgNCs to be a promising possible fluorescent probe for the detection of reactive oxygen/nitrogen species (ROS/RNS) in live cells because red-emitting species are susceptible to oxidation and consequently convert to green-emitting species. Finally, the as-prepared AgNCs were demonstrated to be a sensitive and specific probe for cellular imaging and the monitoring of ROS/RNS levels, which broadens the applications of AgNCs and provides a new tool for related biological investigations.

更新日期：2018-07-12

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