体内微透析在癫痫研究中的用途和未来前景。,ACS Chemical Neuroscience

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体内微透析在癫痫研究中的用途和未来前景。
ACS Chemical Neuroscience ( IF 4.1 ) Pub Date : 2018-07-23 , DOI: 10.1021/acschemneuro.8b00271
Alexander G Zestos ₁ , Hiram Luna-Munguia ₂ , William C Stacey _{3,

4} , Robert T Kennedy _{5,

6}

Affiliation

癫痫病是一种常见的神经系统疾病，其特征是复发性不可预测的癫痫发作。在过去的30年中，微透析采样已用于测量癫痫发作之前，之中和之后兴奋性和抑制性神经递质的浓度变化。这些进展通过识别与癫痫发作相关的神经化学变化并将其与电生理数据相关联，在癫痫研究中取得了突破。方法学的最新进展可能有助于进一步描述癫痫发作的化学基础。已经开发了一种新的致烟现象模型，可以更好地控制与自发性发作相似的发作时间。该模型将有助于在癫痫发作之前和之中进行化学测量。微透析采样的最新进展，包括使用分段流动，“快速”液相色谱法（LC）和带激光诱导的荧光的毛细管电泳（CE-LIF），其时间分辨率显着提高到了1分钟以上，可用于测量瞬时的，自发的神经化学物质与癫痫发作有关的变化。已经开发出显着小于常规探针并允许更大空间分辨率的超细采样探针。它们可能允许靶向对癫痫研究重要的特定大脑区域。将微透析取样与光遗传学和光刺激的神经递质释放耦合，也可能对研究癫痫发作有用。毛细管电泳和激光诱导荧光（CE-LIF）可以将时间分辨率显着提高到1分钟以上，可用于测量与癫痫发作相关的瞬时，自发性神经化学变化。已经开发出显着小于常规探针并允许更大空间分辨率的超细采样探针。它们可能允许靶向对癫痫研究重要的特定大脑区域。将微透析取样与光遗传学和光刺激的神经递质释放耦合，也可能对研究癫痫发作有用。毛细管电泳和激光诱导荧光（CE-LIF）可以将时间分辨率显着提高到1分钟以上，可用于测量与癫痫发作相关的瞬时，自发性神经化学变化。已经开发出显着小于常规探针并允许更大空间分辨率的超细采样探针。它们可能允许靶向对癫痫研究重要的特定大脑区域。将微透析取样与光遗传学和光刺激的神经递质释放耦合，也可能对研究癫痫发作有用。已经开发出显着小于常规探针并允许更大空间分辨率的超细采样探针。它们可能允许靶向对癫痫研究重要的特定大脑区域。将微透析取样与光遗传学和光刺激的神经递质释放耦合，也可能对研究癫痫发作有用。已经开发出显着小于常规探针并允许更大空间分辨率的超细采样探针。它们可能允许靶向对癫痫研究重要的特定大脑区域。将微透析取样与光遗传学和光刺激的神经递质释放耦合，也可能对研究癫痫发作有用。

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Use and Future Prospects of in Vivo Microdialysis for Epilepsy Studies.

Epilepsy is a common neurological disease characterized by recurrent unpredictable seizures. For the last 30 years, microdialysis sampling has been used to measure changes in excitatory and inhibitory neurotransmitter concentrations before, during, and after seizures. These advances have fostered breakthroughs in epilepsy research by identifying neurochemical changes associated with seizures and correlating them to electrophysiological data. Recent advances in methodology may be useful in further delineating the chemical underpinnings of seizures. A new model of ictogenesis has been developed that allows greater control over the timing of seizures that are similar to spontaneous seizures. This model will facilitate making chemical measurements before and during a seizure. Recent advancements in microdialysis sampling, including the use of segmented flow, "fast" liquid chromatography (LC), and capillary electrophoresis with laser-induced fluorescence (CE-LIF) have significantly improved temporal resolution to better than 1 min, which could be used to measure transient, spontaneous neurochemical changes associated with seizures. Microfabricated sampling probes that are markedly smaller than conventional probes and allow for a much greater spatial resolution have been developed. They may allow the targeting of specific brain regions important to epilepsy studies. Coupling microdialysis sampling to optogenetics and light-stimulated release of neurotransmitters may also prove useful for studying epileptic seizures.

更新日期：2018-07-12

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