Characterize Collective Lysosome Heterogeneous Dynamics in Live Cell with a Space- and Time-Resolved Method,Analytical Chemistry

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Characterize Collective Lysosome Heterogeneous Dynamics in Live Cell with a Space- and Time-Resolved Method
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-11 00:00:00 , DOI: 10.1021/acs.analchem.8b01563
Hansen Zhao ₁ , Qiming Zhou ₂ , Mengchan Xia ₁ , Jiaxin Feng ₁ , Yang Chen _{2,

3} , Sichun Zhang ₁ , Xinrong Zhang ₁

Affiliation

While studies of collective cell migration and bacteria swarming have tremendously promoted our fundamental knowledge of the complex systematic phenomena, the quantitative characterization of the collective organelles movement at subcellular level is yet to be fully explored. Here we tagged the lysosomes in live cells with fluorescent probe and imaged their spatial motion with wide field microscopy. To quantitatively characterize the collective lysosomal behavior with high spatiotemporal heterogeneity dynamics, we developed the particle collective analysis (PECAN) method based on the single particle tracking techniques. Thousands of trajectories were detected and analyzed in each single cell. The reliability was validated by comparing with traditional PIV method, simulated and experimental data sets. We show that the lysosomes in live cells move collectively with spatial heterogeneous and temporal long-term correlated dynamics. Furthermore, the continuous wavelet analysis suggested the existence of collective lysosomal oscillation in mouse neural cells. Generally, our method provides a practical workflow for characterizing the collective lysosomal motions which can benefit related areas such as organelles mediated drug delivery and cell activity profiling.

中文翻译：

用空间和时间分辨方法表征活细胞中的集体溶酶体异质动力学

尽管对集体细胞迁移和细菌聚集的研究极大地促进了我们对复杂系统现象的基本认识，但在亚细胞水平上对集体细胞器运动的定量表征尚待充分探索。在这里，我们用荧光探针标记了活细胞中的溶酶体，并用宽视野显微镜对它们的空间运动进行了成像。为了定量表征具有高时空异质性动力学的集体溶酶体行为，我们开发了基于单粒子跟踪技术的粒子集体分析（PECAN）方法。在每个单个单元格中检测并分析了数千条轨迹。通过与传统的PIV方法，模拟和实验数据集进行比较，验证了可靠性。我们显示活细胞中的溶酶体与空间异质性和时间长期相关动力学共同移动。此外，连续小波分析表明小鼠神经细胞中存在集体溶酶体振荡。通常，我们的方法为表征集体溶酶体运动提供了一种实用的工作流程，该过程可以使相关领域受益，例如细胞器介导的药物传递和细胞活性分析。

更新日期：2018-07-11

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