Based on photo-induced electron transfer, 1,8-naphthalic anhydride-based organic molecular fluorescent pH-sensor (1) was designed and synthesized. Hydrophobic, fluorescent hybrid nanosensor (1-PS₃₅-b-PAA₃₀) encapsulated with the hydrophobic pH responsive fluorophore for sensing intracellular pH has been fabricated based on the self-assembly of amphiphilic diblock copolymer PS₃₅-b-PAA₃₀ and 1. The as-synthesized 1-PS₃₅-b-PAA₃₀ sensor exhibits excellent photo-stability, good anti-disturbance ability, and enhanced fluorescence intensity under acidic environment with respect to the corresponding free dye in highly polar aqueous system because of the encapsulation of 1 inside nanoparticle cores with weak polarity environment. The fluorescence intensity of 1-PS₃₅-b-PAA₃₀ is enhanced by 7.3-fold upon changing from base (pH = 9.0) to acid (pH = 4) in aqueous system, which can exactly meet the physiological pH range in cells. Moreover, its linear fluorescent response from pH 5.2 to 7.4 makes this sensor suitable for the practical tracking of pH fluctuation in live cells. The fluorescence imaging and TEM experiments indicated that the sensor permeated into cells and could not be observed because of aggregation in acidic condition.

基于光诱导电子转移，1,8-萘二甲酸酐系有机分子荧光的pH传感器（1）设计并合成。疏水性的，荧光杂交纳米传感器（1-PS ₃₅ - b -PAA ₃₀封装有用于感测细胞内pH疏水pH响应荧光团）已经制造基于所述自组装两亲性嵌段共聚物PS的₃₅ - B- PAA ₃₀和1。合成后的1-PS ₃₅ - b -PAA ₃₀相对于高极性水体系中的相应游离染料，由于在弱极性环境中包封1个内部纳米粒子核，因此该传感器在酸性环境下具有优异的光稳定性，良好的抗干扰能力和增强的荧光强度。1-PS ₃₅ - b -PAA ₃₀的荧光强度在水溶液系统中，从碱（pH = 9.0）变为酸（pH = 4）时，pH值提高了7.3倍，可以完全满足细胞的生理pH范围。此外，其从pH 5.2到7.4的线性荧光响应使该传感器适合实际跟踪活细胞中的pH波动。荧光成像和TEM实验表明，传感器渗透到细胞中，由于在酸性条件下聚集而无法观察到。