Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11,Journal of the American Chemical Society

当前位置： X-MOL 学术 › J. Am. Chem. Soc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2016-01-04 , DOI: 10.1021/jacs.5b11357
Jun-Young Kim _{1,

2,

3} , Srikanta Sahu ₃ , Yin-Hoe Yau ₄ , Xu Wang ₁ , Susana Geifman Shochat ₄ , Per Halkjær Nielsen _{2,

5} , Morten Simonsen Dueholm ₅ , Daniel E. Otzen ₆ , Jungyeol Lee ₁ , May Margarette Salido Delos Santos ₂ , Joey Kuok Hoong Yam _{2,

7} , Nam-Young Kang ₃ , Sung-Jin Park ₃ , Hawyoung Kwon _{1,

2} , Thomas Seviour ₂ , Liang Yang _{2,

4} , Michael Givskov _{2,

8} , Young-Tae Chang _{1,

3}

Affiliation

Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

中文翻译：

用细菌淀粉样蛋白靶向荧光探针 CDy11 检测病原生物膜

细菌生物膜是造成多种持续感染的原因。在临床上，生物膜相关感染的诊断在很大程度上依赖于培养方法，无法检测到不可培养的细菌。用于生物膜成像的新型荧光探针的鉴定将极大地促进病原性细菌感染的诊断。在此，我们报告了一种新型荧光探针 CDy11（指定为黄色 11 的化合物），它通过面向多样性的荧光库方法（DOFLA）靶向铜绿假单胞菌生物膜基质中的淀粉样蛋白。CDy11 被进一步证明可用于铜绿假单胞菌在植入物和角膜感染小鼠模型中的体内成像。

更新日期：2016-01-04

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南