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Biological and Chemical Control of Sclerotinia sclerotiorum using Stachybotrys levispora and Its Secondary Metabolite Griseofulvin
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-26 00:00:00 , DOI: 10.1021/acs.jafc.7b04197 Alany Ingrid Ribeiro 1 , Eveline Soares Costa 1 , Sergio Scherrer Thomasi 2 , Dayson Fernando Ribeiro Brandão 3 , Paulo Cesar Vieira 1 , João Batista Fernandes 1 , Moacir Rossi Forim 1 , Antonio Gilberto Ferreira 1 , Sérgio Florentino Pascholati 3 , Luis Fernando Pascholati Gusmão 4 , Maria Fátima das Graças Fernandes da Silva 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-26 00:00:00 , DOI: 10.1021/acs.jafc.7b04197 Alany Ingrid Ribeiro 1 , Eveline Soares Costa 1 , Sergio Scherrer Thomasi 2 , Dayson Fernando Ribeiro Brandão 3 , Paulo Cesar Vieira 1 , João Batista Fernandes 1 , Moacir Rossi Forim 1 , Antonio Gilberto Ferreira 1 , Sérgio Florentino Pascholati 3 , Luis Fernando Pascholati Gusmão 4 , Maria Fátima das Graças Fernandes da Silva 1
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Sclerotinia sclerotiorum is responsible for the white mold of soybeans, and the difficulty to control the disease in Brazil is causing million-dollar damages. Stachybotrys levispora has shown activity against S. sclerotiorum. In our present investigation, we analyzed the chemical basis of this inhibition. Eight compounds were isolated, and using spectroscopic methods, we identified their structures as the known substances 7-dechlorogriseofulvin, 7-dechlorodehydrogriseofulvin, griseofulvin, dehydrogriseofulvin, 3,13-dihydroxy-5,9,11-trimethoxy-1-methylbenzophenone, griseophenone A, 13-hydroxy-3,5,9,11-tetramethoxy-1-methylbenzophenone, and 12-chloro-13-hydroxy-3,5,9,11-tetramethoxy-1-methylbenzophenone. Griseofulvin inhibited the mycelial growth of S. sclerotiorum at 2 μg mL–1. Thus, the antagonistic effect of S. levispora to S. sclerotiorum may well be due to the presence of griseofulvins. Our results stimulate new work on the biosynthesis of griseofulvins, to locate genes that encode key enzymes in these routes and use them to increase the production of these compounds and thus potentiate the fungicide effect of this fungus. S. levispora represents an agent for biocontrol, and griseofulvin represents a fungicide to S. sclerotiorum.
中文翻译：
利用小孢葡萄穗霉及其次生代谢物灰黄霉素对核盘菌的生物和化学防治
核盘菌是导致大豆白霉病的罪魁祸首，而在巴西，控制该病害的难度正在造成数百万美元的损失。 Stachybotrys levispora已显示出抗核盘菌的活性。在我们目前的研究中，我们分析了这种抑制的化学基础。分离得到8个化合物，采用光谱方法鉴定其结构为已知物质7-脱氯灰黄霉素、7-脱氯脱氢灰黄霉素、灰黄霉素、脱氢灰黄霉素、3,13-二羟基-5,9,11-三甲氧基-1-甲基二苯甲酮、灰苯酮A 、13-羟基-3,5,9,11-四甲氧基-1-甲基二苯甲酮和12-氯-13-羟基-3,5,9,11-四甲氧基-1-甲基二苯甲酮。灰黄霉素在 2 μg mL –1浓度下可抑制核盘菌菌丝生长。因此， S. levispora对S. sclerotiorum的拮抗作用很可能是由于灰黄霉素的存在。我们的结果刺激了灰黄霉素生物合成的新工作，定位编码这些途径中关键酶的基因，并利用它们来增加这些化合物的产量，从而增强这种真菌的杀菌效果。细孢链球菌代表生物防治剂，灰黄霉素代表核盘链球菌的杀真菌剂。
更新日期：2018-06-26
中文翻译：
利用小孢葡萄穗霉及其次生代谢物灰黄霉素对核盘菌的生物和化学防治
核盘菌是导致大豆白霉病的罪魁祸首，而在巴西，控制该病害的难度正在造成数百万美元的损失。 Stachybotrys levispora已显示出抗核盘菌的活性。在我们目前的研究中，我们分析了这种抑制的化学基础。分离得到8个化合物，采用光谱方法鉴定其结构为已知物质7-脱氯灰黄霉素、7-脱氯脱氢灰黄霉素、灰黄霉素、脱氢灰黄霉素、3,13-二羟基-5,9,11-三甲氧基-1-甲基二苯甲酮、灰苯酮A 、13-羟基-3,5,9,11-四甲氧基-1-甲基二苯甲酮和12-氯-13-羟基-3,5,9,11-四甲氧基-1-甲基二苯甲酮。灰黄霉素在 2 μg mL –1浓度下可抑制核盘菌菌丝生长。因此， S. levispora对S. sclerotiorum的拮抗作用很可能是由于灰黄霉素的存在。我们的结果刺激了灰黄霉素生物合成的新工作，定位编码这些途径中关键酶的基因，并利用它们来增加这些化合物的产量，从而增强这种真菌的杀菌效果。细孢链球菌代表生物防治剂，灰黄霉素代表核盘链球菌的杀真菌剂。
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