Resilin-PEG 混合水凝胶产生具有异质微结构的可降解弹性支架,Biomacromolecules

当前位置： X-MOL 学术 › Biomacromolecules › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Resilin-PEG 混合水凝胶产生具有异质微结构的可降解弹性支架
Biomacromolecules ( IF 5.5 ) Pub Date : 2015-12-22 00:00:00 , DOI: 10.1021/acs.biomac.5b01255
Christopher L McGann , Robert E Akins ₁ , Kristi L Kiick ₂

Affiliation

源自节肢弹性蛋白样多肽（RLP）的水凝胶表现出出色的机械弹性和细胞相容性；通过与其他多肽和聚合物缀合来扩展基于 RLP 的材料的多功能性将为一系列材料的设计带来巨大的希望。在这里，我们对由重组 RLP 和多臂 PEG 大分子单体组成的混合水凝胶的生化和机械性能进行了研究。这些杂化水凝胶可以通过 RLP 上半胱氨酸残基的硫醇与多臂 PEG 上的乙烯基砜基团之间的迈克尔型加成反应快速交联。振荡流变学和拉伸测试证实了弹性体水凝胶的形成，其机械弹性可与主动脉弹性蛋白相媲美；水凝胶的硬度可以通过交联比轻松调节。RLP-PEG 水凝胶的大分子相分离具有赋予异质微观结构的独特优势，可通过简单的混合和交联来定位细胞。对基质金属蛋白酶 (MMP) 对 RLP 降解的评估说明了多肽在可溶形式和交联成水凝胶时的特异性蛋白水解作用。最后，人间充质干细胞（hMSC）的成功封装和可行的三维培养证明了RLP-PEG凝胶的细胞相容性。总体而言，RLP-PEG杂化水凝胶的细胞相容性、弹性体机械性能、微观异质性和可降解性为细胞指导性结构化组织工程支架的开发提供了一系列有前景的特性。

"点击查看英文标题和摘要"

Resilin-PEG Hybrid Hydrogels Yield Degradable Elastomeric Scaffolds with Heterogeneous Microstructure

Hydrogels derived from resilin-like polypeptides (RLPs) have shown outstanding mechanical resilience and cytocompatibility; expanding the versatility of RLP-based materials via conjugation with other polypeptides and polymers would offer great promise in the design of a range of materials. Here, we present an investigation of the biochemical and mechanical properties of hybrid hydrogels composed of a recombinant RLP and a multiarm PEG macromer. These hybrid hydrogels can be rapidly cross-linked through a Michael-type addition reaction between the thiols of cysteine residues on the RLP and vinyl sulfone groups on the multiarm PEG. Oscillatory rheology and tensile testing confirmed the formation of elastomeric hydrogels with mechanical resilience comparable to aortic elastin; hydrogel stiffness was easily modulated through the cross-linking ratio. Macromolecular phase separation of the RLP-PEG hydrogels offers the unique advantage of imparting a heterogeneous microstructure, which can be used to localize cells, through simple mixing and cross-linking. Assessment of degradation of the RLP by matrix metalloproteinases (MMPs) illustrated the specific proteolysis of the polypeptide in both its soluble form and when cross-linked into hydrogels. Finally, the successful encapsulation and viable three-dimensional culture of human mesenchymal stem cells (hMSCs) demonstrated the cytocompatibility of the RLP-PEG gels. Overall, the cytocompatibility, elastomeric mechanical properties, microheterogeneity, and degradability of the RLP-PEG hybrid hydrogels offer a suite of promising properties for the development of cell-instructive, structured tissue engineering scaffolds.

更新日期：2015-12-22

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南